Strategies for Electrochemical Point-of-Care Biosensors

Author(s)

Wachholz Junior, Dagwin ; Deroco, Patricia Batista ; Hryniewicz, Bruna M. ; Kubota, Lauro Tatsuo

• Document type: Journal article
• Source: ANNUAL REVIEW OF ANALYTICAL CHEMISTRY; v. 18, p. 27-pg., 2025-01-01.

Abstract

Electrochemical biosensors have emerged as pivotal tools in point-of-care (POC) sensing, offering rapid, sensitive, and cost-effective detection platforms. Different strategies for advancing electrochemical POC biosensors have been explored recently, including fabrication methodologies and advances in biorecognition elements. This review comprehensively explores the miniaturization and integration of portable and wireless devices into fully integrated systems, highlighting recent advancements and challenges in fabrication techniques. We also discuss different enhancement strategies for biorecognition in POC testing, including immunosensors, aptasensors, genosensors, and CRISPR-based biosensors, evaluating their respective strengths and applications. Furthermore, this review addresses the complexity of multiplexing within electrochemical biosensing platforms. Finally, we outline some critical considerations for field deployment and commercialization of electrochemical POC biosensors. We aim to provide a comprehensive overview of advancing electrochemical biosensors toward robust and scalable POC solutions by synthesizing advancements across this emerging field. (AU)

• FAPESP's process: 21/09706-0 - Development of a CRISPR/Cas-based electrochemical biosensor for T-Cell Leukemia virus (HTLV-1) and Staphylococcus Aureus bacteria detection with point-of-care devices
• Grantee: Dagwin Wachholz Júnior
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/22127-2 - Development of novel materials strategic for integrated analytical devices
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/00166-2 - Paper-Based Electrochemical Biosensor for Traumatic Brain Injury detection
• Grantee: Patrícia Batista Deroco
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 23/09451-7 - Development of CRISPR-based biosensors for detection of disease-related genes
• Grantee: Bruna Maria Hryniewicz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants