Nitric oxide detection by electrochemistry selective probe: calibration in the study environment is mandatory

Author(s)

Dos Reis, Roberta Albino ; Boudier, Ariane ; Piquard, Flavian ; Piereti, Joana C. ; Seabra, Amedea B. ; Clarot, Igor

• Document type: Journal article
• Source: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 157, p. 9-pg., 2025-04-25.

Abstract

Nitric oxide (NO) plays a crucial role in various physiological processes, making its detection and controlled release significant for both therapeutic and environmental contexts. Electrochemical sensors are widely used for NO detection due to their high sensitivity and real-time monitoring capabilities. However, challenges such as interference from other gasotransmitters, sensor degradation, and calibration difficulties-especially in complex biological matrices-hinder accurate NO measurement. This review discusses recent advancements in electrochemical NO detection, with a focus on the impact of complex biological matrices, calibration strategies, and sensor designs. The release of NO from nanoparticles, such as S-nitrosoglutathione (GSNO)-encapsulating chitosan nanoparticles, is used as a case study for improving NO detection accuracy. Future innovations in sensor technology and nanoparticle design are expected to expand the applicability of NO detection in personalized medicine and environmental monitoring. (AU)

• FAPESP's process: 22/14645-2 - Nitric oxide releasing hydrogels and vascular prosthesis for cardiovascular applications
• Grantee: Marcelo Ganzarolli de Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 24/12112-2 - Nanoparticles combined with gaseous transmitters for biomedical and agricultural applications
• Grantee: Amedea Barozzi Seabra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 23/16363-7 - Duo-gasotransmitters for biomedical applications: bioformulation of nanoparticles containing Aspirin-NOSH
• Grantee: Roberta Albino dos Reis
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/03646-2 - Impact of nanoplatforms allied to nitric oxide and chemotherapeutics on cytotoxicity and sensitization of resistant tumor cells
• Grantee: Joana Claudio Pieretti
• Support Opportunities: Scholarships in Brazil - Doctorate