| Full text | |
| Author(s): Show less - |
da Silva, Julia Dezanetti
;
Dias, Vinicius Aragao Tejo
;
Rabello, Julia Publio
;
Barrance, Fernanda Angela Correia
;
Teruya, Milena Miyu
;
Taciro, Marilda Keico
;
Jacobus, Ana Paula
;
Leme, Jaci
;
Bernardino, Thaissa Consoni
;
Jorge, Soraia Attie Calil
;
Nunez, Eutimio Gustavo Fernandez
Total Authors: 11
|
| Document type: | Journal article |
| Source: | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 344, p. 11-pg., 2025-07-14. |
| Abstract | |
This work aimed to develop chemometrics models based on multi-way partial least squares (MPLS) in combination with 2D fluorescence spectral data to monitor biochemically the Zika-virus-like particles upstream stage using baculovirus/insect cell expression platform. Four bioreactor experiments using different operation conditions were performed, and 96 samples were collected to obtain spectral and biochemical data. The biochemical parameters under study were glutamate (Glu), lactate (Lac), glutamine (Gln), ammonium (NH4+), and total protein (Tp) concentrations, as well as viable cell density (Xv), cell viability (Cv), and virus titer. Absolute and relative prediction errors were calculated together with their standard deviations. Besides, VLP formation was confirmed by transmission electron microscopy with immunostaining. The relative errors of 3.7 %, 1.5 %, 0.1 %, 2.4 %, 0.007 %, 1.4 %, and 3.2 % were confirmed for Glu, Lac, Gln, Xv, Cv, NH4+, log10 (Vt), and Tp, respectively. These relative errors were lower than those obtained for the same expression system using other spectroscopical techniques like Raman, Near infrared, and Mid-infrared. These results underscore the effectiveness of 2D-fluorescence spectroscopy as a valuable technique for monitoring and optimizing VLP production processes. (AU) | |
| FAPESP's process: | 24/02567-2 - Fluorescence spectroscopy for biochemical monitoring of VLP-Zika production |
| Grantee: | Júlia Dezanetti da Silva |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 22/02713-3 - Establishment of scalable bioprocesses for producing virus-like particles |
| Grantee: | Eutimio Gustavo Fernández Núñez |
| Support Opportunities: | Research Grants - Initial Project |
| FAPESP's process: | 23/09463-5 - Laser wavelength and sample conditioning effects on biochemical monitoring of SARS-CoV-2 VLP production upstream stage by Raman spectroscopy |
| Grantee: | Júlia Públio Rabello |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 23/14085-0 - Online monitoring of the SARS-CoV-2 VLP bioreactor production process by Raman spectroscopy |
| Grantee: | Milena Miyu Teruya |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 23/17480-7 - Optimization of Zika virus VLP production in chemically defined medium using Quality by Design principles |
| Grantee: | Vinícius Aragão Tejo Dias |
| Support Opportunities: | Scholarships in Brazil - Master |