| Full text | |
| Author(s): |
da Costa, Daniel Fernandes
;
Rodrigues, Maira da Silva
;
Borali, Luciana Marino
;
de Souza, Beatriz Marques
;
Habibi, Hamid R.
;
Nobrega, Rafael Henrique
Total Authors: 6
|
| Document type: | Journal article |
| Source: | ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY; v. 117, p. 11-pg., 2025-08-01. |
| Abstract | |
Environmental contaminants BPS and BPAF, emerging substitutes for BPA, are raising concerns as endocrine disruptors. This study assessed their effects on zebrafish spermatogenesis using ex vivo testicular explants exposed to 0.5, 5, or 50 mu g/L of each compound for 7 days. Both chemicals impaired spermatogenesis by reducing meiotic (Spcp) and post-meiotic (Spt) cyst formation, with BPS showing stronger effects. Additionally, they altered the expression of marker genes related to spermatogenesis, steroidogenesis, and epigenetic regulation. Notably, cyp19a1a was upregulated by both, indicating estrogenic activity; however, BPS did not affect estrogen receptor expression, suggesting an indirect antiandrogenic mechanism, while BPAF modulated esr1 and esr2a, indicating direct estrogenic effects. Our findings provide evidence that the dose-response for both compounds was nonmonotonic. These results imply that BPS and BPAF can disrupt reproductive and molecular processes, challenging their safety as BPA substitutes and highlighting the need for further investigation and more stringent environmental regulations. (AU) | |
| FAPESP's process: | 19/22997-3 - Thyroid hormones and the relation to the reproductive-somatotropic axis and their possible role in embryonic development in response to maternal stress in Zebrafish (Danio rerio) |
| Grantee: | Maira da Silva Rodrigues |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 21/13146-0 - Risk assessment of Bisphenol A analogue (BPAF) in zebrafish spermatogenesis and embryonic development |
| Grantee: | Luciana Marino Borali |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 21/06742-5 - New insights on gonadal signals (growth factors and lncRNAs) that regulate gonadal maturation in fish: basic knowledge and development of biotechnological strategies to delay puberty |
| Grantee: | Rafael Henrique Nóbrega |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 23/13211-1 - Regulation of gonadal maturation in fish: role of long non-coding RNAs and microRNAs and their applications in Fish Farming |
| Grantee: | Beatriz Marques de Souza |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |