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Comparison of the toxicity of bisphenol A and bisphenol S individually and in association in HepG2 cells

Grant number: 16/07661-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): October 01, 2016
Effective date (End): September 30, 2018
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal researcher:Fernando Barbosa Júnior
Grantee:Maria Fernanda Hornos Carneiro
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):16/23481-2 - Evaluation of the ability of coenzyme Q10 to counteract the effects upon meiosis and/or early embryogenesis of bisphenol A, dibutyl phthalate, 2-(thiocyanomethylthio)benzothiazole, permethrin, and N,N-diethyl-meta-toluamide in Caenorhabditis elegans, BE.EP.PD

Abstract

Bisphenol A (BPA) is a chemical compound produced in large quantities widely used in the production of plastic, polycarbonates and epoxy resins, in order to make a variety of consumer products. The oral route is the most frequent way of exposure. In addition, BPA is metabolized primarily by the liver being excreted in urine. Studies have shown that human exposure to BPA can lead to obesity and diseases including diabetes, arteriosclerosis, genital malformations, liver disorders and cancers. BPA is known to be an endocrine disruptor with estrogenic activity that may affect the endocrine system and cause hormone-sensitive tumorigenesis. However, the mechanisms by which BPA exerts genotoxicity are still little known. Restrictions and regulations were created to impose limits on the use of this substance, but alternative bisphenol compounds with chemical structure similar and more stable to heat, such as bisphenol S (BPS), have been developed and are being used. Despite a reasonable knowledge of the toxic effects of bisphenol A, little is known about the toxicity of bisphenol S. In addition, there are no restrictions on the use of this compound. It should be noted that although are exposed to a mixture of toxicants, most studies have focused on the toxicological effects of an unique agent excluding possible effect of others in association. Thus, the identification of the potential toxic effect of such mixtures is a relevant topic. Considering BPA and BPS which have been suggested to have a carcinogenic potential, toxicity evaluation per se and in co-exposure is a topic still containing many gaps. The study aims to assess the effect of exposure to the BPA and BPS, individually or in association, upon cytotoxicity, genotoxicity, mutagenicity, apoptosis and DNA oxidative damage in HepG2 cells. For this, HepG2 cells will be exposed to BPA, BPS and BPA + BPS at different concentrations and evaluated for cytotoxicity (MTT assay), genotoxicity (comet assay), mutagenicity (citoma test), apoptosis (flow citometry) and formation of 8-hydroxydeoxyguanosine (ELISA). The results of the study will contribute to the establishment of the toxicological profile of BPA and BPS individually and as a mixture, especially regarding the potential genotoxicity of them and the possible mechanism of oxidative DNA damage.

News published in Agência FAPESP Newsletter about the scholarship:
Un antioxidante revierte el daño contra la fertilidad que provoca la exposición al bisfenol-A 
Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol-A 
Articles published in other media outlets (34 total):
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UOL: Substância reverte dano à fertilidade causado em vermes por bisfenol-A (06/Sep/2020)
UOL: Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol-A (23/Apr/2020)
Central das Notícias: Substância reverte dano à fertilidade causado em vermes por bisfenol-A (06/Sep/2020)
Jornal da USP online: Em laboratório, antioxidante reverte dano à fertilidade causado por material comum em plásticos (03/Sep/2020)
Xw.qq.com (China): Antioxidant reverses damage to fertility caused by exposure to bisphenol A (01/Jun/2020)
What Really Happened: Antioxidant reverses damage to fertility caused by exposure to Bisphenol A (29/May/2020)
Naturopathic Doctor News and Review (EUA) online: CoQ10 Could Help Reduce Harm From BPA (28/May/2020)
NCYT Amazings (Espanha): Un antioxidante revierte el daño contra la fertilidad que provoca la exposición al bisfenol-A (12/May/2020)
Tenemos Noticias (Venezuela): Un antioxidante revierte el daño contra la fertilidad que provoca la exposición al bisfenol-A (12/May/2020)
Nexus Newsfeed (Austrália): Antioxidant reverses damage to fertility caused by exposure to Bisphenol A (11/May/2020)
Santé blog (França): Bisphénol A : La coenzyme Q10 pour rétablir la fertilité (09/May/2020)
Tekk.tv (Alemanha): Antioxidans kehrt die Fruchtbarkeitsschäden um, die durch die Exposition gegenüber Bisphenol A verursacht wurden (08/May/2020)
News Medical (Austrália): Harmful effects of BPA can be reversed by administering coenzyme Q10 (07/May/2020)
Medical Xpress (Reino Unido): Antioxidant reverses damage to fertility caused by exposure to bisphenol A (07/May/2020)
Before It's News (EUA): Antioxidant Reverses Damage to Fertility Caused by Exposure to Bisphenol A (07/May/2020)
Medindia (Índia): Antioxidant Downs Damage Caused by Bisphenol A Exposure (07/May/2020)
Lab Manager (Canadá): Antioxidant Reverses Fertility Damage Caused by BPA Exposure (07/May/2020)
Mindzilla (Reino Unido): Antioxidant Reverses Damage to Fertility Caused by Exposure to Bisphenol A (07/May/2020)
Natural Blaze: Antioxidant Reverses Damage to Fertility Caused by Exposure to Bisphenol A (07/May/2020)
Sound Health and Lasting Wealth (Nigéria): Harmful effects of BPA can be reversed by administering coenzyme Q10 (07/May/2020)
Innovations Report (Alemanha): Antioxidant reverses damage to fertility caused by exposure to bisphenol A (07/May/2020)
Health910: Antioxidant reverses damage to fertility caused by exposure to bisphenol A (07/May/2020)
247 News Around The World: Harmful effects of BPA can be reversed by administering coenzyme Q10 (07/May/2020)
NewsColony (Nigéria): Harmful effects of BPA can be reversed by administering coenzyme Q10 (07/May/2020)
Environmental News Network (EUA): Antioxidant Reverses Damage To Fertility Caused By Exposure To Bisphenol A (06/May/2020)
Bioengineer (Reino Unido): Antioxidant reverses damage to fertility caused by exposure to bisphenol A (06/May/2020)
Science Codex: Antioxidant reverses damage to fertility caused by exposure to bisphenol A (06/May/2020)
Scienmag Science Magazine (Reino Unido): Antioxidant reverses damage to fertility caused by exposure to bisphenol A (06/May/2020)
Fresh Hourly - the latest news: Antioxidant reverses damage to fertility caused by exposure to bisphenol A (06/May/2020)
NewsLab online: Dano à fertilidade causado por exposição ao bisfenol-A pode ser revertido por antioxidante em modelos experimentais (24/Apr/2020)
Atemporal: Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol-A (24/Apr/2020)
Tá Sabendo?: Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol-A. (23/Apr/2020)
Digital Rádio e TV: Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol-A. (23/Apr/2020)
Central das Notícias: Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol (23/Apr/2020)