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Evaluation of the ability of coenzyme Q10 to counteract the effects upon meiosis and/or early embryogenesis of bisphenol A, dibutyl phthalate, 2-(thiocyanomethylthio)benzothiazole, permethrin, and N,N-diethyl-meta-toluamide in Caenorhabditis elegans

Grant number: 16/23481-2
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): May 20, 2017
Effective date (End): May 19, 2018
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:Fernando Barbosa Júnior
Grantee:Maria Fernanda Hornos Carneiro
Supervisor abroad: Monica Paola Colaiacovo
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Local de pesquisa : Harvard University, Boston, United States  
Associated to the scholarship:16/07661-0 - Comparison of the toxicity of bisphenol A and bisphenol S individually and in association in HepG2 cells, BP.PD

Abstract

Endocrine disruptors can mimic natural hormones on their respective receptors leading to adverse effects. Caenorhabditis elegans (C. elegans) is a small nematode which has many instances of conservation of mode of toxic action in comparison to mammals. Preliminary studies of Dr Colaiacovo's group have identified 5 environmental chemicals: bisphenol A (BPA) and dibutyl phthalate (DBP), likely endocrine disruptors frequently used as plasticizers, as well as the pesticides 2-thiocyanomethylthio) benzothiazole (TCMTB), Permethrin, and N,N-diethyl-meta-toluamide (DEET), that result in increased chromosome non-disjunction and meiotic defects in C. elegans. Given the widespread prevalence of endocrine disruptors and pesticides in our environment, it is critically important to understand their impact on reproductive health and identify ways to mitigate their effects and improve fertility and early embryogenesis outcomes. Coenzyme Q10 (CoQ10) has been suggested to improve fertility outcomes, but overall data is still inconclusive. The aim of the study is to determine how BPA, TCMTB, Permethrin, DEET and DBP affect meiosis and/or early embryogenesis and whether CoQ10 can improve reproductive health outcomes. Brood size and embryonic viability of C. elegans will be examined following either continuous exposure to each of these five chemicals starting at eggs or 24- and 42-hour exposures starting at the L4 larval stage. This will be compared to vehicle and CoQ10 alone, to co-exposures (chemical + CoQ10) as well as to chemical exposures for worms starting as eggs followed by CoQ10 exposure starting at the L4 stage. Once a regimen is established that reproducibly exhibits the strongest improvements to embryonic lethality and/or sterility, we will follow strategies described below to examine whether there is an improvement of defects in chromosome morphology and synapsis, double-strand break repair, and increased germ cell apoptosis by using different techniques such as high-resolution microscopy, Fluorescence in situ Hybridization (FISH), RT-PCR, immunostaining, and RNA interference (RNAi).

News published in Agência FAPESP Newsletter about the scholarship:
Antioxidant reverses damage to fertility caused by exposure to bisphenol A 
Articles published in other media outlets (34 total):
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UOL: Substância reverte dano à fertilidade causado em vermes por bisfenol-A (06/Sep/2020)
UOL: Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol-A (23/Apr/2020)
Central das Notícias: Substância reverte dano à fertilidade causado em vermes por bisfenol-A (06/Sep/2020)
Jornal da USP online: Em laboratório, antioxidante reverte dano à fertilidade causado por material comum em plásticos (03/Sep/2020)
What Really Happened: Antioxidant reverses damage to fertility caused by exposure to Bisphenol A (29/May/2020)
Naturopathic Doctor News and Review (EUA) online: CoQ10 Could Help Reduce Harm From BPA (28/May/2020)
NCYT Amazings (Espanha): Un antioxidante revierte el daño contra la fertilidad que provoca la exposición al bisfenol-A (12/May/2020)
Tenemos Noticias (Venezuela): Un antioxidante revierte el daño contra la fertilidad que provoca la exposición al bisfenol-A (12/May/2020)
Nexus Newsfeed (Austrália): Antioxidant reverses damage to fertility caused by exposure to Bisphenol A (11/May/2020)
Santé blog (França): Bisphénol A : La coenzyme Q10 pour rétablir la fertilité (09/May/2020)
Tekk.tv (Alemanha): Antioxidans kehrt die Fruchtbarkeitsschäden um, die durch die Exposition gegenüber Bisphenol A verursacht wurden (08/May/2020)
News Medical (Austrália): Harmful effects of BPA can be reversed by administering coenzyme Q10 (07/May/2020)
Medical Xpress (Reino Unido): Antioxidant reverses damage to fertility caused by exposure to bisphenol A (07/May/2020)
Before It's News (EUA): Antioxidant Reverses Damage to Fertility Caused by Exposure to Bisphenol A (07/May/2020)
Medindia (Índia): Antioxidant Downs Damage Caused by Bisphenol A Exposure (07/May/2020)
Lab Manager (Canadá): Antioxidant Reverses Fertility Damage Caused by BPA Exposure (07/May/2020)
Mindzilla (Reino Unido): Antioxidant Reverses Damage to Fertility Caused by Exposure to Bisphenol A (07/May/2020)
Natural Blaze: Antioxidant Reverses Damage to Fertility Caused by Exposure to Bisphenol A (07/May/2020)
Sound Health and Lasting Wealth (Nigéria): Harmful effects of BPA can be reversed by administering coenzyme Q10 (07/May/2020)
Innovations Report (Alemanha): Antioxidant reverses damage to fertility caused by exposure to bisphenol A (07/May/2020)
Health910: Antioxidant reverses damage to fertility caused by exposure to bisphenol A (07/May/2020)
247 News Around The World: Harmful effects of BPA can be reversed by administering coenzyme Q10 (07/May/2020)
NewsColony (Nigéria): Harmful effects of BPA can be reversed by administering coenzyme Q10 (07/May/2020)
Environmental News Network (EUA): Antioxidant Reverses Damage To Fertility Caused By Exposure To Bisphenol A (06/May/2020)
Bioengineer (Reino Unido): Antioxidant reverses damage to fertility caused by exposure to bisphenol A (06/May/2020)
Science Codex: Antioxidant reverses damage to fertility caused by exposure to bisphenol A (06/May/2020)
Scienmag Science Magazine (Reino Unido): Antioxidant reverses damage to fertility caused by exposure to bisphenol A (06/May/2020)
Fresh Hourly - the latest news: Antioxidant reverses damage to fertility caused by exposure to bisphenol A (06/May/2020)
NewsLab online: Dano à fertilidade causado por exposição ao bisfenol-A pode ser revertido por antioxidante em modelos experimentais (24/Apr/2020)
Atemporal: Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol-A (24/Apr/2020)
Tá Sabendo?: Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol-A. (23/Apr/2020)
Digital Rádio e TV: Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol-A. (23/Apr/2020)
Central das Notícias: Antioxidante reverte dano à fertilidade causado por exposição ao bisfenol (23/Apr/2020)
Harvard Medical School (EUA): Rescue Attempt (06/Feb/2020)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HORNOS CARNEIRO, MARIA FERNANDA; SHIN, NARA; KARTHIKRAJ, RAJENDIRAN; BARBOSA, JR., FERNANDO; KANNAN, KURUNTHACHALAM; COLAIACOVO, MONICA P. Antioxidant CoQ10 Restores Fertility by Rescuing Bisphenol A-Induced Oxidative DNA Damage in the Caenorhabditis elegans Germline. Genetics, v. 214, n. 2, p. 381-395, FEB 2020. Web of Science Citations: 2.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.