| Full text | |
| Author(s): |
Machado, Mauricio J.
;
Jacinavicius, Fernanda R.
;
Medice, Rhuana V.
;
Dextro, Rafael B.
;
Feitosa, Anderson M. T.
;
Weiss, Marcio B.
;
Pellegrinetti, Thierry A.
;
Cotta, Simone R.
;
Crnkovic, Camila M.
;
Fiore, Marli F.
Total Authors: 10
|
| Document type: | Journal article |
| Source: | FRONTIERS IN MICROBIOLOGY; v. 16, p. 14-pg., 2025-07-04. |
| Abstract | |
Introduction Terpenes and terpenoids are vital components in diverse metabolic pathways, forming the terpenome-the complete spectrum of terpene-related compounds biosynthesized by an organism. Integrating bioinformatic tools has significantly enhanced the ability to assess metabolic potential by combining these computational approaches with experimental biochemical data. Furthermore, gene annotation provides critical insights into specialized targets, facilitating the identification of shared or unique features across different strains.Aims and methods This study investigates the presence of terpene compounds in cyanobacterial strains isolated from tropical soda lakes using a combination of gene mining, synteny analysis, phylogenetics, and metabolomics.Results and discussion Key enzymes, including phytoene synthase and squalene hopene cyclase, were identified, showing significant similarities and evolutionary links to gene copies in Cyanobacteria from diverse ecological environments. Metabolomic analysis complemented genomic predictions, uncovering a rich diversity of tetraterpene compounds, particularly carotenoids. Notably, triterpene hopanoids were found exclusively in a unicellular strain. These compounds show significant potential for cellular protection, metabolic adaptation, and biotechnological uses. They might support microbial communities in extreme environments, such as the saline-alkaline lakes of the Pantanal Biome in Brazil, developing unique survival and resilience strategies in these harsh conditions.Conclusion This study highlights the extensive range of insights that can be obtained by integrating genetics and biochemistry in exploring cyanobacterial diversity, especially from organisms thriving in extreme environments. (AU) | |
| FAPESP's process: | 18/24049-2 - BIOGEOGRAPHY OF MICROBIAL COMMUNITIES IN SODA LAKES OF BRAZILIAN PANTANAL |
| Grantee: | Simone Raposo Cotta |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 24/02448-3 - Analysis of the impact of cyanobacteria inoculation on the maize rhizosphere community |
| Grantee: | Fernanda Rios Jacinavicius |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 22/02872-4 - Integrated strategies for natural product discovery from Brazilian cyanobacteria |
| Grantee: | Camila Manoel Crnkovic |
| Support Opportunities: | Research Grants - Initial Project |
| FAPESP's process: | 16/14227-5 - Climatic changes and environmental impacts in wetlands of Pantanal region (Brazil): quantification, control factors and long term temporal modeling |
| Grantee: | Adolpho José Melfi |
| Support Opportunities: | Research Program on Global Climate Change - Thematic Grants |
| FAPESP's process: | 23/08974-6 - Evaluation of agroforest connected with the Amazons soil microbiology |
| Grantee: | Rafael Barty Dextro |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/12644-0 - Microbial communities and functional genes involved in nutrient cycling in Nhecolândia Wetlands (MS) |
| Grantee: | Thierry Alexandre Pellegrinetti |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |