| Full text | |
| Author(s): |
Vilela, Felipe Pinheiro
;
Rodrigues, Dalia dos Prazeres
;
Beiting, Daniel
;
Falcao, Juliana Pfrimer
Total Authors: 4
|
| Document type: | Journal article |
| Source: | Journal of Applied Microbiology; v. 136, n. 8, p. 14-pg., 2025-08-01. |
| Abstract | |
Aims: Analyze the survival and transcriptional response of Salmonella Infantis (S. Infantis) against acid, oxidative, increased osmolarity, and thermal stresses. Methods and results: 25 strains isolated from human, food, and veterinary sources in Brazil between 2013 and 2018 were analyzed. 8 log(10) CFU ml(-1) inoculums were exposed for 10 min, 1 h, or 24 h to acid stress (HCl pH 2.6); oxidative stress (15 mmol l(-1) H2O2); NaCl 9%; refrigeration (4 degrees C); freezing (-20 degrees C); and heating (63/74 degrees C). Most strains (n = 22) survived the acid stress after 10 min and 1 h. Strains maintained high populations under oxidative stress (>87.7%) and NaCl 9% (111.4%). No strains survived heating, while refrigeration prevented multiplication (similar to 101%), and freezing reduced populations (<92.6%). Strain 1143/14 had its transcriptome sequenced under acid and oxidative stresses and NaCl 9%. 14 up-regulated and 38 down-regulated transcripts under acid stress, and 407 up-regulated and 143 down-regulated under oxidative stress were expressed, associated with biological processes of response and adaptation. No transcripts were differentially expressed with NaCl 9%. Conclusions: Salmonella Infantis survival against most stresses represents a challenge for its control. The efficacy of heating reinforced its importance to control Salmonella in food. While acid survival involved the expression of virulence and broad stress response genes, oxidative stress survival showed the expression of genes specific to the adaptation to this condition. (AU) | |
| FAPESP's process: | 21/07365-0 - Genomic, transcriptomic and phenotypic evaluation of virulence aspects of Salmonella Infantis strains isolated from diverse sources in Brazil |
| Grantee: | Felipe Pinheiro Vilela |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 22/07013-0 - Genome analyses, transcriptome, mutants' construction and phenotypic characterization of Campylobacter coli strains isolated from different sources during 25 years in Brazil |
| Grantee: | Juliana Pfrimer Falcão |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 23/11427-7 - Transcriptomic analyses of Salmonella Infantis from Brazil under survival conditions related to human health and food safety |
| Grantee: | Felipe Pinheiro Vilela |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |