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A new synthetic peptide GA-KKALKKLKKALKKAL-CONH2 exhibits antiviral activity against ZIKV in multiple stages of the replicative cycle

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Lima, Maria Leticia Duarte ; Sanches, Paulo Ricardo da Silva ; Geraldini, Dayla Bott ; Ayusso, Gabriela Miranda ; da Conceicao, Pamela Joyce Previdelli ; Carvalho, Tamara ; Gonzalez, Jorge Enrique Hernandez ; Gismene, Carolina ; Bittar, Cintia ; Arni, Raghuvir Krishnaswamy ; Cilli, Eduardo Maffud ; Calmon, Marilia de Freitas ; Rahal, Paula
Total Authors: 13
Document type: Journal article
Source: VIROLOGY; v. 611, p. 12-pg., 2025-07-29.
Abstract

Zika virus (ZIKV) is an emerging arbovirus, and its infection is often asymptomatic or mild; however, it can lead to severe neurological disorders. Currently, there are no approved treatments or vaccines for ZIKV, highlighting the urgent need to explore potential therapeutic options. In this study, we evaluated the antiviral activity of a novel synthetic peptide (GA-peptide) against ZIKV in vitro. The GA-peptide exhibited dose-dependent inhibition of the virus, affecting multiple stages of the ZIKV replication cycle. It demonstrated virucidal activity and effectively protected Vero cells from ZIKV infection. Additionally, the GA-peptide disrupted viral entry by targeting both the attachment and internalization phases, as well as post-entry stages of the infection. In silico analyses identified potential viral targets that interact with the GA-peptide. These findings underscore the GApeptide's promising potential as a therapeutic agent against ZIKV and its relevance in the development of new antiviral drugs. (AU)

FAPESP's process: 22/03901-8 - Computer-guided identification of inhibitors against staphylococcal exfoliative toxins
Grantee:Jorge Enrique Hernández González
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 23/02956-6 - In vitro evaluation of peptides and bioconjugates against Chikungunya Virus (CHIKV) and utilization of murine model for assessing the efficacy and toxicity of hit compounds
Grantee:Paulo Ricardo da Silva Sanches
Support Opportunities: Regular Research Grants
FAPESP's process: 23/04491-0 - Searching for peptide ligands for proteases from Zika, Yellow Fever, Chikungunya and Mayaro arboviruses using Phage Display
Grantee:Dayla Bott Geraldini
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 24/00876-8 - Protein exosites, cryptic sites and moonlighting: identification, functional mapping and effects of changes in structure
Grantee:Carolina Gismene
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 19/07784-3 - In vitro study of the action of synthetic peptides as antivirals against CHIKV
Grantee:Marilia de Freitas Calmon
Support Opportunities: Regular Research Grants
FAPESP's process: 24/13327-2 - Integrating Protein Flexibility into Machine Learning Models for Virtual Hit Identification
Grantee:Jorge Enrique Hernández González
Support Opportunities: Scholarships in Brazil - Support Program for Fixating Young Doctors
FAPESP's process: 20/05761-3 - Study of the action of synthetic peptides as antivirals against SARS-CoV-2 (COVID-19) and combined evaluation with commercial anti-inflammatories
Grantee:Eduardo Maffud Cilli
Support Opportunities: Regular Research Grants
FAPESP's process: 22/05411-8 - Development of peptide/guanidine bioconjugates with anti-Leishmania activity
Grantee:Eduardo Maffud Cilli
Support Opportunities: Regular Research Grants
FAPESP's process: 20/08615-8 - Protein exosites, cryptic sites and moonlighting: identification, functional mapping and effects of changes in structure
Grantee:Raghuvir Krishnaswamy Arni
Support Opportunities: Research Projects - Thematic Grants