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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream

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Caltabiano, Caroline [1] ; Maximo, Felipe Rodrigues [2] ; Spadari, Ana Paula Pimentel [2] ; da Conceicao Miranda, Daniel Duarte ; Pivatto Serra, Marcia Milena [1] ; Ribeiro, Marcelo Lima [1] ; Real Martinez, Carlos Augusto [1]
Total Authors: 7
[1] Univ Sao Francisco, Sch Med, Postgrad Program Hlth Sci, BR-12900000 Braganca Paulista, SP - Brazil
[2] Univ Sao Francisco, Hosp Med, BR-12900000 Braganca Paulista, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Digestive Diseases and Sciences; v. 56, n. 4, p. 1037-1046, APR 2011.
Web of Science Citations: 13

No studies have evaluated the effectiveness of 5-ASA against oxidative DNA damage in experimental models of diversion colitis. To evaluate the effects of 5-ASA against oxidative DNA damage in an experimental model of diversion colitis. Twenty-six Wistar rats were divided into two groups corresponding to sacrifice at 2 or 4 weeks after fecal diversion of the left colon by means of proximal colostomy and distal mucosa fistula. Each group was divided into two subgroups according to intervention in excluded colon performed with 0.9% saline solution or 5-ASA. Level of oxidative DNA damage was determined by comet assay in cells obtained from segments with and without fecal stream before and after H(2)O(2) challenge. For statistical analysis, was used one-way analysis of variance (ANOVA), adopting a 5% significance level (P < 0.05). Levels of DNA damage were always higher in colon segments without fecal stream, regardless of the intervention solution employed. DNA damage in colon segments with and without fecal stream in animals irrigated with 5-ASA was lower when compared with those treated with saline solution, regardless of time of irrigation. These levels remained lower after intervention with 5-ASA, even after H(2)O(2) challenge. Enema with 5-ASA reduces oxidative DNA damage in epithelial cells of colon segments without fecal stream, even after H(2)O(2) challenge, confirming the effects of 5-ASA against DNA damage by oxygen free radicals. (AU)

FAPESP's process: 06/02306-6 - Analisys of correlation among histological aspects, p53 expression and DNA damage in mucosal cells from animals submited to diversion colitis
Grantee:Carlos Augusto Real Martinez
Support type: Regular Research Grants