| Full text | |
| Author(s): |
Kamezawa, Joao G. Veneziani
;
Kato, Lilian Seiko
;
Fonseca, Elisania Kelly Barbosa
;
Arruda, Marco Aurelio Zezzi
Total Authors: 4
|
| Document type: | Journal article |
| Source: | BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY; v. 12, n. 49, p. 8-pg., 2025-10-01. |
| Abstract | |
Tissue and cell culture techniques have become pivotal in plant biotechnology, particularly for the improvement and mass propagation of economically important species such as soybean (Glycine max). Within this context, somatic embryogenesis has emerged as a powerful tool. Micronutrients like Fe, Mn, Mo, and Zn play essential roles in cellular metabolism and can serve as indicators of tissue growth and development. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) is widely recognized for its sensitivity in multielement analysis at trace levels; however, sample preparation remains a critical and often resource-intensive step. This study aims to develop and compare three distinct sample preparation methods for the determination of micronutrients in soybean calli using ICP-MS: (1) conventional acid decomposition, (2) acid extraction, and (3) acidless decomposition. The results indicated that all three methods provided statistically equivalent concentrations for all evaluated elements (p >= 0.05), with values ranging from 0.54 +/- 0.08 mu g g-1 for Mo to 14 +/- 1 mu g g-1 for Mn. Additionally, the residual chemical content (RCC) in the proposed methods was below the detection limit of 8 mg L-1. Sustainability assessment using the AGREEprep tool highlighted acidless decomposition as the most environmentally friendly method, primarily due to its use of greener reagents and higher sample throughput. These findings suggest that acidless decomposition presents a viable and sustainable alternative for micronutrient analysis in plant tissue culture applications. (AU) | |
| FAPESP's process: | 21/15218-8 - Nanometallomics applied to soybean cultivation |
| Grantee: | Lilian Seiko Kato |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 22/13166-3 - Nanoionomics applied to soybean cultivation |
| Grantee: | João Gabriel Veneziani Kamezawa |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis |
| Grantee: | Marco Aurelio Zezzi Arruda |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/24445-8 - EMU granted in process 2018/25207-0: inductively coupled plasma mass spectrometer |
| Grantee: | Marco Aurelio Zezzi Arruda |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 18/25207-0 - Nanometallomics applied to soybean cultivation for studying the effects of metal-related nanoparticles |
| Grantee: | Marco Aurelio Zezzi Arruda |
| Support Opportunities: | Research Projects - Thematic Grants |