| Full text | |
| Author(s): |
Tollentino, Fernanda M.
[1, 2]
;
Polotto, Milena
[1, 2]
;
Nogueira, Mauricio L.
[1]
;
Lincopan, Nilton
[3]
;
Neves, Patricia
[3]
;
Mamizuka, Elsa M.
[3]
;
Remeli, Gisele A.
[1]
;
De Almeida, Margarete T. G.
[1]
;
Rubio, Fernando G.
[1]
;
Nogueira, Mara C. L.
[1]
Total Authors: 10
|
| Affiliation: | [1] Fac Med Sao Jose do Rio Preto, Dept Doencas Dermatol Infecciosas & Parasitarias, Microbiol Lab, BR-15090000 Sao Jose Do Rio Preto - Brazil
[2] Univ Estadual Paulista, Dept Biol, Sao Jose Do Rio Preto - Brazil
[3] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | MICROBIAL DRUG RESISTANCE; v. 17, n. 1, p. 7-16, MAR 2011. |
| Web of Science Citations: | 35 |
| Abstract | |
The aim of this study was to investigate the presence and prevalence of bla(TEM), bla(SHV), and bla(CTX-M) and bla(GES)-like genes, responsible for extended spectrum beta-lactamases (ESBLs) production in clinical isolates of Klebsiella pneumoniae collected from a Brazilian tertiary care hospital. Sixty-five ESBL producing K. pneumoniae isolates, collected between 2005 and 2007, were screened by polymerase chain reaction (PCR). Identification of bla genes was achieved by sequencing. Genotyping of ESBL producing K. pneumoniae was performed by the enterobacterial repetitive intergenic consensus-PCR with cluster analysis by the Dice coefficient. The presence of genes encoding ESBLs was confirmed in 59/65 (90.8%) isolates, comprising 20 bla(CTX-M-2), 14 bla(CTX-M-59), 12 bla(CTX-M-15), 9 bla(SHV-12), 1 bla(SHV-2), 1 bla(SHV-2a), 1 bla(SHV-5), and 1 bla(SHV-31) genes. The ESBL genes bla(SHV-12), bla(SHV-31), and bla(CTX-M-15), and the chromosome-encoded SHV-type beta-lactamase capable of hydrolyzing imipenem were detected in Brazil for the first time. The analysis of the enterobacterial repetitive intergenic consensus-PCR band patterns revealed a high rate of multiclonal bla(CTX-M) carrying K. pneumoniae isolates (70.8%), suggesting that dissemination of encoding plasmids is likely to be the major cause of the high prevalence of these genes among the K. pneumoniae isolates considered in this study. (AU) | |
| FAPESP's process: | 06/00514-0 - Genotypic analysis of blaSHV of Klebsiella pneumoniae isolated in the Hospital de Base of São José do Rio Preto |
| Grantee: | Mara Corrêa Lelles Nogueira |
| Support Opportunities: | Regular Research Grants |