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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Utilization of pineapple stem juice to enhance enzyme-hydrolytic efficiency for sugarcane bagasse after an optimized pre-treatment with alkaline peroxide

Full text
Author(s):
Monte, J. R. [1] ; Brienzo, M. [1] ; Milagres, A. M. F. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Dept Biotechnol, Sch Engn Lorena, BR-12602810 Lorena, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: APPLIED ENERGY; v. 88, n. 1, p. 403-408, JAN 2011.
Web of Science Citations: 26
Abstract

The enzymatic hydrolysis of sugarcane bagasse was investigated by treating a peroxide-alkaline bagasse with a pineapple stem juice, xylanase and cellulase. Pre-treatment procedures of sugarcane bagasse with alkaline hydrogen peroxide were evaluated and compared. Analyses were performed using 2(4) factorial designs, with pre-treatment time, temperature, magnesium sulfate and hydrogen peroxide concentration as factors. The responses evaluated were the yield of cellobiose and glucose released from pretreated bagasse after enzymatic hydrolysis. The results show that the highest enzymatic conversion was obtained for bagasse using 2% hydrogen peroxide at 60 degrees C for 16 h in the presence of 0.5% magnesium sulfate. Bagasse (5%) was treated with pineapple stem extract, which contains mixtures of protease and esterase, in combination with xylanase and cellulase. It was observed that the amount of glucose and cellobiose released from bagasse increased with the mixture of enzymes. It is believed that the enzymes present in pineapple extracts are capable of hydrolyze specific linkages that would facilitate the action of digesting plant cell walls enzymes. This increases the amount of glucose and other hexoses that are released during the enzymatic treatment and also reduces the amount of cellulase necessary in a typical hydrolysis. (C) 2010 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 08/03204-8 - Isolation of polysacharides from sugar cane bagasse to obtain oligosacharides using enzymatic hydrolysis and gel permeation chromatography
Grantee:Adriane Maria Ferreira Milagres
Support Opportunities: Regular Research Grants