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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantitative Detection of Enterotoxigenic Bacteroides fragilis Subtypes Isolated from Children with and without Diarrhea

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Author(s):
Merino, V. R. C. [1] ; Nakano, V. [1] ; Liu, C. [2] ; Song, Y. [2] ; Finegold, S. M. [2, 3, 4] ; Avila-Campos, M. J. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Anaerobe Lab, Dept Microbiol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] VA Med Ctr W Los Angeles, Los Angeles, CA - USA
[3] UCLA Sch Med, Dept Med, Los Angeles, CA - USA
[4] UCLA Sch Med, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA - USA
Total Affiliations: 4
Document type: Journal article
Source: Journal of Clinical Microbiology; v. 49, n. 1, p. 416-418, JAN 2011.
Web of Science Citations: 13
Abstract

A rapid real-time PCR ( RT-PCR) approach was developed to detect the bft gene subtypes in Bacteroides fragilis isolated from fecal samples. DNA obtained from diarrhea (110) and nondiarrhea (150) samples was evaluated. Subtype 1 was observed in 9 (8.2%) diarrhea and 7 (4.7%) nondiarrhea samples. Subtype 2 was not detected in any DNA samples, and subtype 3 was observed in only 1 diarrhea sample. The presence of the bft-1 gene did not show any statistically significant differences between the groups of children. This technique could be used to evaluate a possible correlation between disease and the presence of B. fragilis enterotoxin. (AU)

FAPESP's process: 07/03655-7 - Quantitative evaluation of Bacteroides fragilis enterotoxigenic strains and respective BFT gene subtypes in immunocompetent children with and without acute diarrhea
Grantee:Victor Rafael Castillo Merino
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)