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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Low-level laser therapy (LLLT; 780 nm) acts differently on mRNA expression of anti- and pro-inflammatory mediators in an experimental model of collagenase-induced tendinitis in rat

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Author(s):
Pires, Debora [1] ; Xavier, Murilo [2] ; Araujo, Tiago [3] ; Silva, Jr., Jose Antnio [3] ; Aimbire, Flavio [2] ; Albertini, Regiane [2, 3]
Total Authors: 6
Affiliation:
[1] Inst Res & Dev, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[2] Univ Camilo Castelo Branco UNICASTELO, Inst Biomed Engn, BR-12247000 Sao Jose Dos Campos, SP - Brazil
[3] UNINOVE, Dept Rehabil Sci, BR-235 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Lasers in Medical Science; v. 26, n. 1, p. 85-94, JAN 2011.
Web of Science Citations: 76
Abstract

Low-level laser therapy (LLLT) has been found to produce anti-inflammatory effects in a variety of disorders. Tendinopathies are directly related to unbalance in expression of pro- and anti-inflammatory cytokines which are responsible by degeneration process of tendinocytes. In the current study, we decided to investigate if LLLT could reduce mRNA expression for TNF-alpha, IL-1 beta, IL-6, TGF-beta cytokines, and COX-2 enzyme. Forty-two male Wistar rats were divided randomly in seven groups, and tendinitis was induced with a collagenase intratendinea injection. The mRNA expression was evaluated by real-time PCR in 7th and 14th days after tendinitis. LLLT irradiation with wavelength of 780 nm required for 75 s with a dose of 7.7 J/cm(2) was administered in distinct moments: 12 h and 7 days post tendinitis. At the 12 h after tendinitis, the animals were irradiated once in intercalate days until the 7th or 14th day in and them the animals were killed, respectively. In other series, 7 days after tendinitis, the animals were irradiated once in intercalated days until the 14th day and then the animals were killed. LLLT in both acute and chronic phases decreased IL-6, COX-2, and TGF-beta expression after tendinitis, respectively, when compared to tendinitis groups: IL-6, COX-2, and TGF-beta. The LLLT not altered IL-1 beta expression in any time, but reduced the TNF-alpha expression; however, only at chronic phase. We conclude that LLLT administered with this protocol reduces one of features of tendinopathies that is mRNA expression for pro-inflammatory mediators. (AU)