A flow injection procedure based on solenoid micro-pumps for spectrophotometric determination of free glycerol in biodiesel

Author(s)

Silva, Sidnei G. ^[1] ; Rocha, Fabio R. P. ^[2]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ctr Energia Nucl Agr, BR-13400970 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: Talanta; v. 83, n. 2, p. 559-564, DEC 15 2010.
• Web of Science Citations: 20

Abstract

A flow system designed with solenoid micro-pumps is proposed for fast and greener spectrophotometric determination of free glycerol in biodiesel. Glycerol was extracted from samples without using organic solvents. The determination involves glycerol oxidation by periodate, yielding formaldehyde followed by formation of the colored (3,5-diacetil-1,4-dihidrolutidine) product upon reaction with acetylacetone. The coefficient of variation, sampling rate and detection limit were estimated as 1.5% (20.0 mg L(-1) glycerol, n =10), 34 h(-1), and 1.0 mg L(-1) (99.7% confidence level), respectively. A linear response was observed from 5 to 50 mg L(-1), with reagent consumption estimated as 345 mu g of KIO(4) and 15 mg of acetylacetone per determination. The procedure was successfully applied to the analysis of biodiesel samples and the results agreed with the batch reference method at the 95% confidence level. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 08/01045-0 - Multicommuted flow systems for determination of free glycerol, manganese and total organic carbon in different sample matrices
• Grantee: Sidnei Gonçalves da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 08/57808-1 - National Institute of Advanced Analytical Science and Technology
• Grantee: Celio Pasquini
• Support Opportunities: Research Projects - Thematic Grants