Characterization of 12 microsatellite loci from an enriched genomic library in polyploid Tibouchina pulchra Cogn. (Melastomataceae)

Author(s)

Brito, Vinicius L. G. ^[1] ; Vigna, Bianca B. Z. ^[2] ; de Souza, Anete P. ^{[2, 1]}

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Plant Biol Dept DBV, BR-13083875 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Genet Engn & Mol Biol Ctr, BR-13083875 Campinas, SP - Brazil

• Document type: Journal article
• Source: CONSERVATION GENETICS RESOURCES; v. 2, n. 1, p. 193-196, DEC 2010.
• Web of Science Citations: 2

Abstract

Tibouchina pulchra is a common hermaphroditic canopy tree of the Melastomataceae in the Brazilian Atlantic Rain Forest that is important for bee communities, reforestation, soil and air bio-indication and urban ornamentation. Twelve polymorphic microsatellite markers were isolated and characterized in 89 genotypes of T. pulchra from two populations of Ubatuba and Sao Luis do Paraitinga municipalities (Sao Paulo, Brazil). The number of alleles observed for each locus ranged from 4 to 31 with an average of 12 alleles per locus. The polymorphism information content varied between 0.51 and 0.92 (average 0.74) and the discriminating power (D) ranged from 0.66 to 0.99 (average 0.87). These microsatellite markers will be useful for assessing genetic studies, conservation management, and ecological and phylogenetic evaluations. (AU)

• FAPESP's process: 03/12595-7 - Floristic composition, structure and functioning of the Dense Rainforest nuclei of Picinguaba and Santa Virgínia of Parque Estadual da Serra do Mar, State of São Paulo, Brazil
• Grantee: Carlos Alfredo Joly
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants