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Development of microsatellite loci for genetic diversity characterization of annatto (Bixa orellana) accessions

Grant number: 11/05243-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): January 31, 2013
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal Investigator:Elizabeth Ann Veasey
Grantee:Gabriel Dequigiovanni
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil


The aim of this project is to characterize the genetic diversity of 63 accessions of annatto (Bixa orellana), maintained at the Germplasm Bank of the Instituto Agronômico (IAC), using microsatellite markers. Microsatellite loci will be developed for the species through an enriched genomic library. Subsequently, the loci obtained will be used to characterize the accessions. We intend to estimate, for the groups of genotypes originating from three Brazilian regions (North, Southeast and Midwest), the allelic and genotypic frequencies, genetic diversity indices such as number of alleles per locus, percentage of polymorphic loci, average observed and expected heterozygosity, Wright's fixation index and Nei's diversity index, verifying the level of genetic diversity of the collection and distribution of variability among and within regions. A cluster analysis will be conducted calculating the distance estimates based on sharing of alleles, resulting in a dissimilarity matrix for individual accessions to view the accessions that are genetically more distant, which will allow the selection of divergent parents to be use in breeding programs. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DEQUIGIOVANI, GABRIEL; FERREYRA RAMOS, SANTIAGO LINORIO; ALVES-PEREIRA, ALESSANDRO; FABRI, ELIANE GOMES; NOGUEIRA CARVALHO, PAULO ROBERTO; DA SILVA, MARTA GOMES; VILELA NOGUEIRA ABDO, MARIA TERESA; MELLO MARTINS, ANTONIO LUCIO; CLEMENT, CHARLES ROLAND; VEASEY, ELIZABETH ANN. Genetic diversity and structure in a major Brazilian annatto (Bixa orellana) germplasm bank revealed by microsatellites and phytochemical compounds. Genetic Resources and Crop Evolution, v. 64, n. 7, p. 1775-1788, . (11/10357-8, 12/08307-5, 11/05243-3, 13/08884-5)
DEQUIGIOVANNI, G.; RAMOS, S. L. F.; ZUCCHI, M. I.; BAJAY, M. M.; PINHEIRO, J. B.; FABRI, E. G.; BRESSAN, E. A.; VEASEY, E. A.. Isolation and characterization of microsatellite loci for Bixa orellana, an important source of natural dyes. Genetics and Molecular Research, v. 13, n. 4, p. 9097-9102, . (12/08307-5, 11/05243-3, 13/08884-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
DEQUIGIOVANNI, Gabriel. Development of microsatellite loci to characterize the genetic diversity of annatto (Bixa orellana L.) accessions. 2013. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.

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