Genetic structure and mating system of local varieties and wild populations of annatto (Bixa orellana L.) in Brazilian Amazonia and Central Brazil using microsatellite markers

• Grant number: 12/08307-5
• Support Opportunities: Regular Research Grants
• Duration: August 01, 2012 - January 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Elizabeth Ann Veasey
• Grantee: Elizabeth Ann Veasey
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Charles Roland Clement ; Doriane Picanço Rodrigues ; Eduardo de Andrade Bressan ; Eliane Gomes Fabri ; José Baldin Pinheiro ; Maria Imaculada Zucchi ; Maria Teresa Gomes Lopes

Abstract

The annatto (Bixa orellana L.) has great economic importance because it is the only source of natural pigment, bixin, used as a natural dye in the pharmaceutical, textile, dairy, food, beverage, paint and cosmetic industries. This project aims to study the genetic diversity and structure, as well as the reproductive system of local varieties and wild populations of annatto in Brazil. For that, microsatellite primers will be developed for the species by means of a microsatellite-enriched genomic library. Local varieties of annatto will be collected along two axes in the Amazon Basin - north-south (Rio Mamore, Madeira, Negro and White) and west-east (Rio Solimões and Amazonas), in the surroundings of Belém, in Central Brasil (Cuiabá - Porto Velho), as well as wild populations collected in the states of Amazonas, Rondônia and Roraima, to detect possible centers of diversity. The mating system will be studied with a hierarchical design of fruit and individuals from wild and cultivated populations of annatto. Various parameters of genetic diversity and population structure will be estimated. Bayesian analyses, plus cluster and principal coordinate analyses will be conducted in order to evaluate the relationship among the accessions and populations. For the reproductive system study, 10 plants will be used of a wild population and a local variety population, where 20 progenies will be evaluated per plant. From this analysis multilocus and single locus outcrossing rates will be estimated, as well as other related parameters. Information about genetic diversity and structure and the reproductive system will help to guide future prospections for breeding programs, as well as plan for the in situ and ex situ conservation of annatto in Brazil. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DEQUIGIOVANI, GABRIEL; FERREYRA RAMOS, SANTIAGO LINORIO; ALVES-PEREIRA, ALESSANDRO; FABRI, ELIANE GOMES; NOGUEIRA CARVALHO, PAULO ROBERTO; DA SILVA, MARTA GOMES; VILELA NOGUEIRA ABDO, MARIA TERESA; MELLO MARTINS, ANTONIO LUCIO; CLEMENT, CHARLES ROLAND; VEASEY, ELIZABETH ANN. Genetic diversity and structure in a major Brazilian annatto (Bixa orellana) germplasm bank revealed by microsatellites and phytochemical compounds. Genetic Resources and Crop Evolution, v. 64, n. 7, p. 1775-1788, OCT 2017. (11/10357-8, 12/08307-5, 11/05243-3, 13/08884-5)

ALVES-PEREIRA, ALESSANDRO; CLEMENT, CHARLES R.; PICANCO-RODRIGUES, DORIANE; VEASEY, ELIZABETH ANN; DEQUIGIOVANNI, GABRIEL; FERREYRA RAMOS, SANTIAGO LINORIO; PINHEIRO, JOSE BALDIN; DE SOUZA, ANETE PEREIRA; ZUCCHI, MARIA IMACULADA. A population genomics appraisal suggests independent dispersals for bitter and sweet manioc in Brazilian Amazonia. EVOLUTIONARY APPLICATIONS, v. 13, n. 2, OCT 2019. (12/08307-5, 18/00036-9, 13/08884-5, 13/00003-0)

DEQUIGIOVANNI, GABRIEL; FERREYRA RAMOS, SANTIAGO LINORIO; GOMES LOPES, MARIA TERESA; CLEMENT, CHARLES ROLAND; RODRIGUES, DORIANE PICANCO; FABRI, ELIANE GOMES; ZUCCHI, MARIA IMACULADA; VEASEY, ELIZABETH ANN. New microsatellite loci for annatto (Bixa orellana), a source of natural dyes from Brazilian Amazonia. Crop Breeding and Applied Biotechnology, v. 18, n. 1, p. 116-122, JAN-MAR 2018. (15/26837-0, 12/08307-5, 13/08884-5)

MOREIRA, PRISCILA AMBROSIO; LINS, JULIANA; DEQUIGIOVANNI, GABRIEL; VEASEY, ELIZABETH ANN; CLEMENT, CHARLES R.. The Domestication of Annatto (Bixa orellana) from Bixa urucurana in Amazonia. ECONOMIC BOTANY, v. 69, n. 2, p. 127-135, JUN 2015. (12/08307-5)

DEQUIGIOVANNI, G.; RAMOS, S. L. F.; ZUCCHI, M. I.; BAJAY, M. M.; PINHEIRO, J. B.; FABRI, E. G.; BRESSAN, E. A.; VEASEY, E. A.. Isolation and characterization of microsatellite loci for Bixa orellana, an important source of natural dyes. Genetics and Molecular Research, v. 13, n. 4, p. 9097-9102, 2014. (12/08307-5, 11/05243-3, 13/08884-5)

ALVES-PEREIRA, ALESSANDRO; ZUCCHI, MARIA IMACULADA; CLEMENT, CHARLES R.; GOMES VIANA, JOAO PAULO; PINHEIRO, JOSE BALDIN; VEASEY, ELIZABETH ANN; DE SOUZA, ANETE PEREIRA. elective signatures and high genome-wide diversity in traditional Brazilian manioc (Manihot esculenta Crantz) varietie. SCIENTIFIC REPORTS, v. 12, n. 1, JAN 24 2022. (12/08307-5, 13/00003-0, 18/00036-9)

ALVES-PEREIRA, ALESSANDRO; CLEMENT, CHARLES R.; PICANCO-RODRIGUES, DORIANE; VEASEY, ELIZABETH A.; DEQUIGIOVANNI, GABRIEL; RAMOS, SANTIAGO L. F.; PINHEIRO, JOSE B.; ZUCCHI, MARIA I.. Patterns of nuclear and chloroplast genetic diversity and structure of manioc along major Brazilian Amazonian rivers. ANNALS OF BOTANY, v. 121, n. 4, p. 625-639, MAR 2018. (13/00003-0, 12/08307-5, 13/11137-7, 13/08884-5)

DEQUIGIOVANNI, GABRIEL; FERREYRA RAMOS, SANTIAGO LINORIO; ALVES-PEREIRA, ALESSANDRO; FABRI, ELIANE GOMES; PICANCO-RODRIGUES, DORIANE; CLEMENT, CHARLES ROLAND; GEPTS, PAUL; VEASEY, ELIZABETH ANN. Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia. PLoS One, v. 13, n. 6, JUN 6 2018. (15/26837-0, 12/08307-5, 16/05912-6, 13/08884-5)

ALVES-PEREIRA, ALESSANDRO; NOVELLO, MARIANA; DEQUIGIOVANNI, GABRIEL; PINHEIRO, JOSE BALDIN; BRANCALION, PEDRO H. S.; VEASEY, ELIZABETH ANN; CLEMENT, CHARLES R.; DE SOUZA, ANETE PEREIRA; ZUCCHI, MARIA IMACULADA. Genomic Diversity of Three Brazilian Native Food Crops Based on Double-Digest Restriction Site-Associated DNA Sequencing. TROPICAL PLANT BIOLOGY, v. 12, n. 4, p. 268-281, DEC 2019. (12/08307-5, 18/00036-9, 13/17354-0, 13/08884-5, 13/00003-0)