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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protein aggregation containing beta-amyloid, alpha-synuclein and hyperphosphorylated tau in cultured cells of hippocampus, substantia nigra and locus coeruleus after rotenone exposure

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Chaves, Rodrigo S. [1] ; Melo, Thaiany Q. [1] ; Martins, Stephanie A. [1] ; Ferrari, Merari F. R. [1]
Total Authors: 4
[1] Univ Sao Paulo, Sch Med, Dept Neurol, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: BMC NEUROSCIENCE; v. 11, NOV 10 2010.
Web of Science Citations: 23

Background: Protein aggregates containing alpha-synuclein, beta-amyloid and hyperphosphorylated tau are commonly found during neurodegenerative processes which is often accompanied by the impairment of mitochondrial complex I respiratory chain and dysfunction of cellular systems of protein degradation. In view of this, we aimed to develop an in vitro model to study protein aggregation associated to neurodegenerative diseases using cultured cells from hippocampus, locus coeruleus and substantia nigra of newborn Lewis rats exposed to 0.5, 1, 10 and 25 nM of rotenone, which is an agricultural pesticide, for 48 hours. Results: We demonstrated that the proportion of cells in culture is approximately the same as found in the brain nuclei they were extracted from. Rotenone at 0.5 nM was able to induce alpha-synuclein and beta amyloid aggregation, as well as increased hyperphosphorylation of tau, although high concentrations of this pesticide (over 1 nM) lead cells to death before protein aggregation. We also demonstrated that the 14kDa isoform of alpha-synuclein is not present in newborn Lewis rats. Conclusion: Rotenone exposure may lead to constitutive protein aggregation in vitro, which may be of relevance to study the mechanisms involved in idiopathic neurodegeneration. (AU)

FAPESP's process: 09/00010-0 - mRNA analysis of mitochondrial transporting and anchoring proteins before the aggregation of proteins involved in neurodegeneration
Grantee:Stephanie Alves Martins
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 09/02345-0 - Study of protein expression related to anterograde transport of mitochondria before protein aggregation involved in neurodegeneration
Grantee:Thaiany Quevedo Melo
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 08/04480-9 - Analysis of intracellular trafficking of mitochondria before protein aggregation formation in neurodegeneration
Grantee:Merari de Fátima Ramires Ferrari
Support type: Regular Research Grants
FAPESP's process: 08/04655-3 - Retrograde transport and anchoring of mitochondria in neuronal culture from hippocampus, substantia nigra and locus coeruleus before the formation on protein aggregates involved in neurodegeneration
Grantee:Rodrigo dos Santos Chaves
Support type: Scholarships in Brazil - Scientific Initiation