Study of autophagy as the driving mechanism of neurodegenerative diseases
| Grant number: | 16/04409-9 |
| Support type: | Scholarships in Brazil - Master |
| Effective date (Start): | August 01, 2016 |
| Effective date (End): | January 31, 2019 |
| Field of knowledge: | Biological Sciences - Morphology - Cytology and Cell Biology |
| Principal Investigator: | Merari de Fátima Ramires Ferrari |
| Grantee: | Raquel de Souza Lima |
| Home Institution: | Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated scholarship(s): | 17/24722-6 - Chaperones machines to combat protein aggregation in Parkinson's Disease, BE.EP.MS |
Abstract Aging is a worldwide issue and the understanding of the cellular events associated to this process are important for the study of age-related diseases, like in neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. The presence of misfolded proteins are common during the neurodegenerative process, and can contribute to organelle dysfunction culminating in cell death. In view of this, the purpose of the present study is to evaluate autophagy process during the course of sporadic neurodegenerative diseases. In view of this, it is proposed to evaluate the protein levels of Beclin-1, LC3I and LC3II, associated with the recruitment and maintenance of the autophagosomes, respectively, and autophagy flux, in culture cells of hippocampus, substantia nigra and locus coeruleus exposed to rotenone as a model of protein aggregation. Also it is intended to analyze the role of the co-chaperon BAG-2, that transits between the lysosomal and proteasome systems, to modulate the autophagic activity and protein aggregation. | |