The bacterial diversity in a Brazilian non-disturbed mangrove sediment

Author(s)

Dias, Armando C. F. ^{[1, 2]} ; Andreote, Fernando D. ^{[3, 4]} ; Rigonato, Janaina ^[1] ; Fiore, Marli Fatima ^[1] ; Melo, Itamar S. ^[3] ; Araujo, Welington Luiz ^[5]

Affiliation

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr, CENA USP, Piracicaba - Brazil

^[2] Univ Sao Paulo, Biotechnol Res Ctr, ICB IV, Sao Paulo - Brazil

^[3] CNPMA Embrapa Meio Ambiente, Lab Environm Microbiol, Jaguariuna - Brazil

^[4] ESALQ Univ Sao Paulo, Dept Soil Sci, Piracicaba - Brazil

^[5] Univ Mogi das Cruzes, Biotechnol Res Ctr, Lab Mol Biol & Microbial Ecol, NIB, BR-08780970 Mogi Das Cruzes, SP - Brazil

• Document type: Journal article
• Source: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY; v. 98, n. 4, p. 541-551, NOV 2010.
• Web of Science Citations: 35

Abstract

The bacterial diversity present in sediments of a well-preserved mangrove in Ilha do Cardoso, located in the extreme south of So Paulo State coastline, Brazil, was assessed using culture-independent molecular approaches (denaturing gradient gel electrophoresis (DGGE) and analysis of 166 sequences from a clone library). The data revealed a bacterial community dominated by Alphaproteobacteria (40.36% of clones), Gammaproteobacteria (19.28% of clones) and Acidobacteria (27.71% of clones), while minor components of the assemblage were affiliated to Betaproteobacteria, Deltaproteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. The clustering and redundancy analysis (RDA) based on DGGE were used to determine factors that modulate the diversity of bacterial communities in mangroves, such as depth, seasonal fluctuations, and locations over a transect area from the sea to the land. Profiles of specific DGGE gels showed that both dominant ('universal' Bacteria and Alphaproteobacteria) and low-density bacterial communities (Betaproteobacteria and Actinobacteria) are responsive to shifts in environmental factors. The location within the mangrove was determinant for all fractions of the community studied, whereas season was significant for Bacteria, Alphaproteobacteria, and Betaproteobacteria and sample depth determined the diversity of Alphaproteobacteria and Actinobacteria. (AU)

• FAPESP's process: 07/08354-5 - Assessment of the genetic diversity of cyanobacteria from São Paulo State mangroves using DGGE and library of 16S rDNA gene
• Grantee: Janaina Rigonato
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 04/13910-6 - Biodiversity and functional activities of microorganisms from mangrove of the State of São Paulo
• Grantee: Itamar Soares de Melo
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants