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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Association of haplotypes in the IL8 gene with susceptibility to chronic periodontitis in a Brazilian population

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Author(s):
Kim, Yeon Jung [1] ; Viana, Aline Cavalcante [1] ; Curtis, Karen Maria C. [2] ; Perez Orrico, Silvana Regina [1] ; Cirelli, Joni Augusto [1] ; Mendes-Junior, Celso Teixeira [3] ; Scarel-Caminaga, Raquel Mantuaneli
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, Dept Diagnost & Cirurgia, Sao Paulo, SP - Brazil
[2] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, Dept Morfol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Clinica Chimica Acta; v. 411, n. 17-18, p. 1264-1268, SEP 6 2010.
Web of Science Citations: 17
Abstract

Background: Interleukin 8 (IL-8) is a chemokine related to the initiation and amplification of acute and chronic inflammatory processes. Polymorphisms in the IL8 gene have been associated with inflammatory diseases. We investigated whether the - 845(T/C) and - 738(T/A) single nucleotide polymorphisms (SNPs) in the IL8 gene, as well as the haplotypes they form together with the previously investigated -353(A/T), are associated with susceptibility to chronic periodontitis. Methods: DNA was extracted from buccal epithelial cells of 400 Brazilian individuals (control n =182, periodontitis n=218). SNPs were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Disease associations were analyzed by the chi(2) test, Exact Fisher test and Clump program. Haplotypes were reconstructed using the expectation-maximization algorithm and differences in haplotype distribution between the groups were analyzed to estimate genetic susceptibility for chronic periodontitis development. Results: When analyzed individually, no SNPs showed different distributions between the control and chronic periodontitis groups. Although, nonsmokers carrying the TTA/CAT (OR = 2.35, 95% CI = 1.03-5.36) and TAT/CTA (OR= 6.05, 95% CI = 1.32-27.7) haplotypes were genetically susceptible to chronic periodontitis. The ITT/TAA haplotype was associated with protection against the development of periodontitis (for nonsmokers OR= 0.22, 95% CI = 0.10-0.46). Conclusion: Although none of the investigated SNPs in the IL8 gene was individually associated with periodontitis, some haplotypes showed significant association with susceptibility to, or protection against, chronic periodontitis in a Brazilian population. (C) 2010 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 05/03231-7 - Investigation of polymorphisms in the promoter region of interleukin 8 in Brazilian individuals with chronic periodontitits
Grantee:Yeon Jung Kim
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 03/10424-0 - Investigation of polymorphisms in the promoter region of interleukin 8 in Brazilian individuals with chronic periodontitits
Grantee:Raquel Mantuaneli Scarel Caminaga
Support Opportunities: Regular Research Grants