Supercritical fluid extraction of lycopene from tomato juice and characterization of its antioxidation activity

Author(s)

Egydio, Juliana A. ^[1] ; Moraes, Angela M. ^[1] ; Rosa, Paulo T. V. ^[2]

Affiliation

^[1] Univ Estadual Campinas, Sch Chem Engn, Dept Biotechnol Proc, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, Dept Phys Chem, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF SUPERCRITICAL FLUIDS; v. 54, n. 2, p. 159-164, AUG 2010.
• Web of Science Citations: 38

Abstract

A new method to extract lycopene from tomato juice using supercritical CO(2) as solvent and without the need to dry the raw material is presented. To conduct the extraction, the tomato juice was subjected to cycles of centrifugation followed by rinsing with absolute ethanol to partially remove the water present in the solid part of the juice. The influence of the temperature and pressure on the extraction efficiency and on the extract antioxidant activity was studied using a factorial experimental design. The extraction efficiency varied from 7.7% to 76.7% and only extraction temperature had a statically significant effect on the process. The reversed phase HPLC analysis showed that lycopene is the major compound of the extract. The extract that presented higher antioxidant activity was obtained at 40 degrees C and 350 bar with 12.7 mmol L(-1) Trolox/g of extract using the DPPH radical scavenging method and 61.3 mmol L(-1) Trolox/g of extract using the rubrene singlet oxygen quenching method. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 06/04243-1 - Lycopene recovery from tomato and watermelon juices using supercritical chromatography
• Grantee: Paulo de Tarso Vieira e Rosa
• Support Opportunities: Regular Research Grants