Neutral Aminopeptidase and Dipeptidyl Peptidase IV Activities in Plasma of Monosodium Glutamate Obese and Food-deprived Rats

Author(s)

Alponti, Rafaela F. ^{[1, 2]} ; Silveira, Paulo F. ^[1]

Affiliation

^[1] Inst Butantan, Pharmacol Lab, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Neurosci & Behav, Sao Paulo - Brazil

• Document type: Journal article
• Source: OBESITY; v. 18, n. 7, p. 1312-1317, JUL 2010.
• Web of Science Citations: 9

Abstract

Biometric parameters, glycemia and activity levels of plasma neutral aminopeptidase (APN) and dipeptidyl peptidase IV (DPPIV) were measured in monosodium glutamate obese and food-deprived rats (MSG-FD), to analyze the involvement of these enzymes in such situations. Plasma APN was distinguished as sensitive (PSA) (K(m) = 7.8 x 10(-5) mol/l) and predominantly insensitive (APM) (K(m) = 21.6 x 10(-5) mol/l) to puromycin, whereas DPPIV was sensitive (DPPIV-DS) (K(m) = 0.24 x 10(-5) mol/l) and predominantly insensitive (DPPIV-DI) (K(m) = 7.04 x 10(-5) mol/l) to diprotin A. Although unchanged in the MSG and food-deprived animals, APM activity levels were closely correlated with body mass, Lee index, and mass of retroperitoneal fat pad in the food deprived, but not in the MSG animals. DPPIV-DI activity levels decreased by 33% and were correlated with body mass, Lee index, and mass of periepididymal fat pad in the food-deprived MSG rats. These data suggest that APM and DPPIV-DI are respectively related to the downregulation of somatostatin in food-deprived rats, and to the recovery of energy balance in MSG obese rats during food deprivation. (AU)

• FAPESP's process: 05/04699-2 - Hypothalamic and hippocampal neutral aminopeptidase (APN/CD13), prolyl dipeptidyl aminopeptidase IV (DPPIV/CD26) and C-FOS in monosodium glutamate-treated and food-deprived rats
• Grantee: Paulo Flávio Silveira
• Support Opportunities: Regular Research Grants