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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Geranylation of benzoic acid derivatives by enzymatic extracts from Piper crassinervium (Piperaceae)

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Author(s):
Lopez, Silvia Noeli [1] ; Lopes, Adriana Aparecida [1] ; Batista, Jr., Joao Marcos [1] ; Flausino, Jr., Otavio [1] ; Bolzani, Vanderlan da Silva [1] ; Kato, Massuo Jorge [2] ; Furlan, Maysa [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista, UNESP, Dept Quim Organ, Inst Quim, NuBBE Nucleo Bioensaios Biossintese & E, BR-14800900 Araraquara, SP - Brazil
[2] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Bioresource Technology; v. 101, n. 12, p. 4251-4260, JUN 2010.
Web of Science Citations: 16
Abstract

The ability to carry out geranylations on aromatic substrates using enzymatic extracts from the leaves of Piper crassinervium (Piperaceae) was evaluated. A literature analysis pointed out its importance as a source of prenylated bioactive molecules. The screening performed on aromatic acceptors (benzoic acids, phenols and phenylpropanoids) including geranyl diphosphate as prenyl donor, showed the biotransformation of the 3,4-dihydroxybenzoic acid by the crude extract, and the p-hydroxybenzoic acid by both the microsomal fraction and the crude extract, after treating leaves with glucose. The analysis of the products allowed the identification of C- and O-geranylated derivatives, and the protease (subtilisin and pepsin) inhibition performed on the O-geranylated compounds showed weak inhibition. Electrophoretic profiles indicated the presence of bands/spots among 56-58 kDa and pI 6-7, which are compatible with prenyltransferases. These findings show that P. crassinervium could be considered as a source of extracts with geranyltransferase activity to perform biotransformations on aromatic substrates. (C) 2010 Elsevier Ltd. All rights reserved. (AU)