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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of secondary palate requires strict regulation of ECM remodeling: sequential distribution of RECK, MMP-2, MMP-3, and MMP-9

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Cardoso de Oliveira Demarchi, Ana Claudia [1] ; Zambuzzi, Willian Fernando [1] ; Silva Paiva, Katiucia Batista [2] ; da Silva-Valenzuela, Maria das Gracas [3] ; Nunes, Fabio Daumas [2] ; Savio Figueira, Rita de Cassia [3] ; Sasahara, Regina Maki [3] ; Almeida Demasi, Marcos Angelo [3] ; Brochado Winnischofer, Sheila Maria [3] ; Sogayar, Mari Cleide [3, 4] ; Granjeiro, Jose Mauro [5, 4]
Total Authors: 11
[1] Univ Estadual Campinas, Dept Biochem, IB, Campinas, SP - Brazil
[2] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, Lab Mol Pathol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Biochem, Inst Chem, Sao Paulo - Brazil
[4] Univ Sao Paulo, Cell & Mol Therapy Ctr, Sao Paulo - Brazil
[5] Univ Fed Fluminense, Inst Biol, Dept Cell & Mol Biol, BR-24020150 Niteroi, RJ - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Cell and Tissue Research; v. 340, n. 1, p. 61-69, APR 2010.
Web of Science Citations: 15

We have evaluated RECK (reversion-inducing-cysteine-rich protein with Kazal motifs), MMP-2 (matrix metalloproteinase-2), MMP-3, and MMP-9 involvement during palate development in mice by using various techniques. Immunohistochemical features revealed the distribution of RECK, MMP-2, and MMP-3 in the mesenchymal tissue and in the midline epithelial seam at embryonic day 13 (E13), MMPs-2, -3, and -9 being particularly expressed at E14 and E14.5. In contrast, RECK was weakly immunostained at these times. Involvement of MMPs was validated by measuring not only their protein expression, but also their activity (zymograms). In situ hybridization signal (ISH) for RECK transcript was distributed in mesenchymal and epithelial regions within palatal shelves at all periods evaluated. Importantly, the results from ISH analysis were in accord with those obtained by real-time polymerase chain reaction. The expression of RECK was found to be temporally regulated, which suggested possible roles in palatal ontogeny. Taken together, our results clearly show that remodeling of the extracellular matrix is finely modulated during secondary palate development and occurs in a sequential manner. (AU)

FAPESP's process: 01/10707-7 - Molecular bases of the control of cell proliferation and origin of neoplasms in the genomic and proteomic era
Grantee:Mari Cleide Sogayar
Support type: Research Projects - Thematic Grants