| Full text | |
| Author(s): |
Berra, Carolina Maria
[1]
;
Martins Menck, Carlos Frederico
[1]
;
Martinez, Glaucia Regina
[2]
;
de Oliveira, Carla Santos
[3]
;
Baptista, Mauricio da Silva
[4]
;
Di Mascio, Paolo
[4]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Univ Fed Parana, Setor Ciencias Biol, Dept Bioquim & Biol Mol, BR-81531990 Curitiba, Parana - Brazil
[3] Univ Fed Mato Grosso do Sul, Ctr Ciencias Biol & Saude, Dept Morfofisiol, BR-79070900 Campo Grande, MS - Brazil
[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05599970 Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Química Nova; v. 33, n. 2, p. 279-283, 2010. |
| Web of Science Citations: | 8 |
| Abstract | |
To investigate oxidative lesions and strand breaks induction by singlet molecular oxygen (¹O2), supercoiled-DNA plasmid was treated with thermo-dissociated DHPNO2 and photoactivated-methylene blue. DNA lesions were detected by Fpg that cleaves DNA at certain oxidized bases, and T4-endoV, which cleaves DNA at cyclobutane pyrimidine dimers and apurinic/apyrimidinic (AP) sites. These cleavages form open relaxed-DNA structures, which are discriminated from supercoiled-DNA. DHPNO2 or photoactivated-MB treatments result in similar plasmid damage profile: low number of single-strand breaks or AP-sites and high frequency of Fpg-sensitive sites; confirming that base oxidation is the main product for both reactions and that ¹O2 might be the most likely intermediate that reacts with DNA. (AU) | |