Performance of IFAT, ELISA, direct parasitological examination and PCR on lymph node aspirates for canine visceral leishmaniasis diagnosis

Author(s)

Camargo, J. B. ^[1] ; Langoni, H. ^[1] ; Troncarelli, M. Z. ^[1] ; Machado, J. G. ^[1] ; Lucheis, S. B. ^{[1, 2]} ; Padovani, C. R. ^[3]

Affiliation

^[1] UNESP Univ Estadual Paulista, Sao Paulo State Univ, Sch Vet Med & Anim Husb, Dept Vet Hyg & Publ Hlth, Botucatu, SP - Brazil

^[2] APTA SAA, Sao Paulo Agcy Agribusiness Technol, Bauru, SP - Brazil

^[3] UNESP Univ Estadual Paulista, Sao Paulo State Univ, Dept Biostat, Botucatu Biosci Inst, Botucatu, SP - Brazil

• Document type: Journal article
• Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 16, n. 3, p. 414-420, 2010.
• Web of Science Citations: 4

Abstract

Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA and PCR. (AU)

• FAPESP's process: 06/06565-6 - Contribution for the diagnostic briefing in canine visceral leishmaniasis as a tool for epidemiologic monitoring and control of this disease
• Grantee: Helio Langoni
• Support Opportunities: Regular Research Grants