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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of dynamic solid phase DNA extraction from blood with magnetically controlled silica beads

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Author(s):
Duarte, Gabriela R. M. [1, 2, 3] ; Price, Carol W. [3] ; Littlewood, Janice L. [3] ; Haverstick, Doris M. [4] ; Ferrance, Jerome P. [3] ; Carrilho, Emanuel [1, 2] ; Landers, James P. [3, 5, 4]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil
[2] Inst Nacl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP - Brazil
[3] Univ Virginia, Dept Chem, Charlottesville, VA 22904 - USA
[4] Univ Virginia, Hlth Sci Ctr, Dept Pathol, Charlottesville, VA 22908 - USA
[5] Univ Virginia, Dept Mech Engn, Charlottesville, VA 22904 - USA
Total Affiliations: 5
Document type: Journal article
Source: ANALYST; v. 135, n. 3, p. 531-537, 2010.
Web of Science Citations: 30
Abstract

A novel solid phase extraction technique is described where DNA is bound and eluted from magnetic silica beads in a manner where efficiency is dependent on the magnetic manipulation of the beads and not on the flow of solution through a packed bed. The utility of this technique in the isolation of reasonably pure, PCR-amplifiable DNA from complex samples is shown by isolating DNA from whole human blood, and subsequently amplifying a fragment of the beta-globin gene. By effectively controlling the movement of the solid phase in the presence of a static sample, the issues associated with reproducibly packing a solid phase in a microchannel and maintaining consistent flow rates are eliminated. The technique described here is rapid, simple, and efficient, allowing for recovery of more than 60% of DNA from 0.6 mu L of blood at a concentration which is suitable for PCR amplification. In addition, the technique presented here requires inexpensive, common laboratory equipment, making it easily adopted for both clinical point-of-care applications and on-site forensic sample analysis. (AU)