| Full text | |
| Author(s): |
Santos, M. O.
[1]
;
Sassaki, R. P.
[1]
;
Ferreira, T. H. S.
[1]
;
Resende, R. M. S.
[2]
;
Chiari, L.
[2]
;
Karia, C. T.
[3]
;
Faleiro, F. G.
[3]
;
Jungmann, L.
[2]
;
Zucchi, M. I.
[4]
;
Souza, A. P.
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP - Brazil
[2] Empresa Brasileira Pesquisa Agropecuaria Embrapa, BR-79002970 Campo Grande, MS - Brazil
[3] Empresa Brasileira Pesquisa Agropecuaria Embrapa, BR-73310970 Planaltina, DF - Brazil
[4] Polo Apta Ctr Rod, BR-13400970 Piracicaba, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | CONSERVATION GENETICS RESOURCES; v. 1, n. 1, p. 481-485, DEC 2009. |
| Web of Science Citations: | 6 |
| Abstract | |
Microsatellites were developed for Stylosanthes macrocephala, aiming at developing tools for studying the genetic diversity of this species. A total of 13 polymorphic microsatellite markers were isolated from a S. macrocephala enriched genomic library. The isolated microsatellites were characterized in 20 accessions of the S. macrocephala germplasm collection belonging to the Empresa Brasileira de Pesquisa Agropecuaria (Embrapa) Cerrados. The number of alleles per locus varied from 2 to 11, with an average of 4 alleles per locus. The observed and expected heterozygosities ranged from 0 to 0.25 and 0.05 to 0.90, respectively. Cross-amplification of the S. macrocephala polymorphic microsatellites was evaluated in three other Stylosanthes species. The microsatellites reported herein are the first set of microsatellite markers developed for S. macrocephala and are potentially useful for further studies on genetic diversity, conservation and breeding of this species. (AU) | |