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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

PIP4KIIA and beta-globin: transcripts differentially expressed in reticulocytes and associated with high levels of Hb H in two siblings with Hb H disease

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Author(s):
Wenning, Marcia R. S. C. [1] ; Mello, Maricilda P. [2] ; Andrade, Tiago G. [3] ; Lanaro, Carolina [3] ; Albuquerque, Dulcineia M. [3] ; Saad, Sara T. O. [3] ; Costa, Fernando F. [3] ; Sonati, Maria F. [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Campinas, Dept Clin Pathol, Sch Med Sci, UNICAMP, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Mol Biol & Genet Ctr, CBMEG, UNICAMP, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Hematol & Hemotherapy Ctr, UNICAMP, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: EUROPEAN JOURNAL OF HAEMATOLOGY; v. 83, n. 5, p. 490-493, NOV 2009.
Web of Science Citations: 7
Abstract

We are reporting here the results of differential gene expression experiments comparing two siblings, a 21-yr-old male and a 19-yr-old female, with the same alpha-thalassemia genotype (-alpha 3.7/--SEA) and quite different levels of Hb H in the peripheral blood (18.7 and 5%, respectively). By using mRNA differential-display reverse-transcription-PCR and suppression subtractive hybridization, two main transcripts were selected in both procedures and validated by qRT-PCR, one corresponding to the phosphatidylinositol phosphate 4-kinase type II-alpha (PIP4KIIA) gene and the other to the beta-globin gene, both over expressed in the patient with the higher percentage of Hb H. Type II PIP kinases produce phosphatidylinositol 4,5 biphosphate, a critical and pleiotropic regulatory molecule involved in diverse cellular activities, including gene expression. Our results suggest that PIP4KIIA may be one of the factors related to the regulation of the beta-globin gene expression and the different levels of Hb H in alpha-thalassemic patients. (AU)

FAPESP's process: 02/13801-7 - Hereditary hemoglobin disorders: molecular genetics, clinical features and animal models with the production of transgenic animals
Grantee:Fernando Ferreira Costa
Support Opportunities: Research Projects - Thematic Grants