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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular cloning and characterization of a ripening-induced polygalacturonase related to papaya fruit softening

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Fabi, Joao Paulo [1] ; Cordenunsi, Beatriz Rosana [1] ; Seymour, Graham B. [2] ; Lajolo, Franco M. [1] ; Oliveira do Nascimento, Joao Roberto [1]
Total Authors: 5
[1] Univ Sao Paulo, FCF, Dept Alimentos & Nutr Expt, Lab Quim Bioquim & Biol Mol Alimentos, BR-05508900 Sao Paulo - Brazil
[2] Univ Nottingham, Plant Sci Div, Loughborough LE12 5RD, Leics - England
Total Affiliations: 2
Document type: Journal article
Source: Plant Physiology and Biochemistry; v. 47, n. 11-12, p. 1075-1081, NOV-DEC 2009.
Web of Science Citations: 28

Pulp softening is one of the most remarkable changes during ripening of papaya (Carica papaya) fruit and it is a major cause for post-harvest losses. Although cell wall catabolism has a major influence on papaya fruit, quality information on the gene products involved in this process is limited. A full-length polygalacturonase cDNA (cpPG) was isolated from papaya pulp and used to study gene expression and enzyme activity during normal and ethylene-induced ripening and after exposure of the fruit to 1-MCP. Northern-blot analysis demonstrated that cpPG transcription was strongly induced during ripening and was highly ethylene-dependent. The accumulation of cpPG transcript was paralleled by enzyme activity, and inversely correlated to the pulp firmness. Preliminary in silica analysis of the cpPG genomic sequence revealed the occurrence of putative regulatory motifs in the promoter region that may help to explain the effects of plant hormones and non-abiotic stresses on papaya fruit firmness. This newly isolated cpPG is an important candidate for functional characterization and manipulation to control the process of pulp softening during papaya ripening. (C) 2009 Elsevier Masson SAS. All rights reserved. (AU)

FAPESP's process: 02/12452-9 - Carbohydrate metabolism during fruit ripening: a functional genomics approach
Grantee:Franco Maria Lajolo
Support type: Research Projects - Thematic Grants