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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic variability of Brazilian isolates of Alternaria alternata detected by AFLP and RAPD techniques

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Author(s):
Dini-Andreote, Francisco [1] ; Pietrobon, Vivian Cristina [1] ; Andreote, Fernando Dini [1] ; Romao, Aline Silva [1] ; Sposito, Marcel Bellato [2] ; Araujo, Welington Luiz [3, 1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Piracicaba, SP - Brazil
[2] Fundo Defesa Citricultura, Dept Cient, Araraquara, SP - Brazil
[3] Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, BR-08780911 Mogi Das Cruzes, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Journal of Microbiology; v. 40, n. 3, p. 670-677, SEP 2009.
Web of Science Citations: 6
Abstract

The Alternaria brown spot (ABS) is a disease caused in tangerine plants and its hybrids by the fungus Alternaria alternata f. sp. citri which has been found in Brazil since 2001. Due to the recent occurrence in Brazilian orchards, the epidemiology and genetic variability of this pathogen is still an issue to be addressed. Here it is presented a survey about the genetic variability of this fungus by the characterization of twenty four pathogenic isolates of A. alternata f. sp. citri from citrus plants and four endophytic isolates from mango (one Alternaria tenuissima and three Alternaria arborescens). The application of two molecular markers Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) had revealed the isolates clustering in distinct groups when fingerprintings were analyzed by Principal Components Analysis (PCA). Despite the better assessment of the genetic variability through the AFLP, significant modifications in clusters components were not observed, and only slight shifts in the positioning of isolates LRS 39/3 and 25M were observed in PCA plots. Furthermore, in both analyses, only the isolates from lemon plants revealed to be clustered, differently from the absence of clustering for other hosts or plant tissues. Summarizing, both RAPD and AFLP analyses were both efficient to detect the genetic variability within the population of the pathogenic fungus Alternaria spp., supplying information on the genetic variability of this species as a basis for further studies aiming the disease control. (AU)