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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative chromosome mapping of repetitive sequences. Implications for genomic evolution in the fish, Hoplias malabaricus

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Author(s):
Cioffi, Marcelo B. [1] ; Martins, Cesar [2] ; Bertollo, Luiz A. C. [1]
Total Authors: 3
Affiliation:
[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13560 Sao Carlos, SP - Brazil
[2] UNESP, Inst Biociencias, Dept Morfol, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BMC GENETICS; v. 10, JUL 7 2009.
Web of Science Citations: 45
Abstract

Background: Seven karyomorphs of the fish, Hoplias malabaricus (A-G) were previously included in two major groups, Group I (A, B, C, D) and Group II (E, F, G), based on their similar karyotype structure. In this paper, karyomorphs from Group I were analyzed by means of distinct chromosomal markers, including silver-stained nucleolar organizer regions (Ag-NORs) and chromosomal location of repetitive sequences (18S and 5S rDNA, and satellite 5SHindIII-DNA), through fluorescence in situ hybridization (FISH), in order to evaluate the evolutionary relationships among them. Results: The results showed that several chromosomal markers had conserved location in the four karyomorphs. In addition, some other markers were only conserved in corresponding chromosomes of karyomorphs A-B and C-D. These data therefore reinforced and confirmed the proposed grouping of karyomorphs A-D in Group I and highlight a closer relationship between karyomorphs A-B and C-D. Moreover, the mapping pattern of some markers on some autosomes and on the chromosomes of the XY and X(1)X(2)Y systems provided new evidence concerning the possible origin of the sex chromosomes. Conclusion: The in situ investigation of repetitive DNA sequences adds new informative characters useful in comparative genomics at chromosomal level and provides insights into the evolutionary relationships among Hoplias malabaricus karyomorphs. (AU)

FAPESP's process: 07/05565-5 - Chromosome markers in Hoplias malabaricus (Characiformes, Erythrinidae): comparative cytogenetics among cytotypes
Grantee:Marcelo de Bello Cioffi
Support Opportunities: Scholarships in Brazil - Master