| Full text | |
| Author(s): |
Total Authors: 3
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| Affiliation: | [1] Univ Sao Paulo, Dept Alimentos & Nutr Expt, FCF, Lab Quim Bioquim & Biol Mol Alimentos, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 1
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| Document type: | Journal article |
| Source: | Scientia Horticulturae; v. 121, n. 2, p. 159-165, JUN 17 2009. |
| Web of Science Citations: | 12 |
| Abstract | |
Papaya (Carica papaya) is a relevant tropical crop and physico-chemical changes take place very quickly, as a consequence of activation of biochemical pathways by de nova synthesis of several proteins. Thus, in order to have information on the changes in gene expression in ripening papaya, transcripts from the pulp of unripe and ripe fruit were profiled by differential-display RT-PCR (DDRT-PCR). Seventy transcript derived fragments (TDFs) isolated from gels were re-amplified by PCR and differential expression of 40 papaya genes was confirmed by reverse northern blotting. Twenty-nine positively cloned TDFs were sequenced, and 17 were putatively identified by homology search. Ten of these genes were downregulated during ripening and UDP-glucose glucosyltransferase, alpha-2 importin, RNase L inhibitor-like protein, and a syntaxin protein were identified. Among the up-regulated genes there was a carboxylesterase, an integral membrane Yip1 family protein, a glycosyl hydrolase family-like protein and an endopolygalacturonase. Considering their relatedness to papaya quality, the fragments of genes potentially implicated in carbohydrate metabolism and pulp softening may be considered of interest for further studies. According to the results, differential display was a feasible approach to investigate differences in gene expression during fruit ripening, and can provide interesting information about those fruits whose genomic data is scarce, as is the case of papayas. (c) 2009 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 02/12452-9 - Carbohydrate metabolism during fruit ripening: a functional genomics approach |
| Grantee: | Franco Maria Lajolo |
| Support Opportunities: | Research Projects - Thematic Grants |