Rat forming incisor requires a rigorous ECM remodeling modulated by MMP/RECK balance

Author(s)

Silva Paiva, Katiucia Batista ^[1] ; Zambuzzi, Willian Fernando ^[2] ; Accorsi-Mendonca, Thais ^[3] ; Taga, Rumio ^[4] ; Nunes, Fabio Daumas ^[1] ; Sogayar, Mari Cleide ^{[5, 6]} ; Granjeiro, Jose Mauro ^{[6, 7]}

Affiliation

^[1] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, Lab Mol Pathol, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Biochem, Lab Signaling Transduct, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, SP - Brazil

^[4] Univ Sao Paulo, Bauru Dent Sch, Dept Biol Sci, Bauru - Brazil

^[5] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Cell & Mol Therapy Ctr, Sao Paulo - Brazil

^[7] Univ Fed Fluminense, Inst Biol, Dept Cell & Mol Biol, BR-24020150 Ctr Niteroi, RJ - Brazil

• Document type: Journal article
• Source: Journal of Molecular Histology; v. 40, n. 3, p. 201-207, JUN 2009.
• Web of Science Citations: 9

Abstract

Reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) is a single membrane-anchored MMP-regulator and regulates matrix metalloproteinases (MMP) 2, 9 and 14. In turn, MMPs are endopeptidases that play a pivotal role in remodeling ECM. In this work, we decided to evaluate expression pattern of RECK in growing rat incisor during, specifically focusing out amelogenesis process. Based on different kinds of ameloblasts, our results showed that RECK expression was conducted by secretory and post-secretory ameloblasts. At the secretory phase, RECK was localized in the infra-nuclear region of the ameloblast, outer epithelium, near blood vessels, and in the stellate reticulum. From the transition to the maturation phases, RECK was strongly expressed by non-epithelial immuno-competent cells (macrophages and/or dendritic-like cells) in the papillary layer. From the transition to the maturation stage, RECK expression was increased. RECK mRNA was amplified by RT-PCR from whole enamel organ. Here, we verified the presence of RECK mRNA during all stages of amelogenesis. These events were governed by ameloblasts and by non-epithelial cells residents in the enamel organ. Concluding, we found differential expression of MMPs-2, -9 and RECK in the different phases of amelogenesis, suggesting that the tissue remodeling is rigorously controlled during dental mineralization. (AU)

• FAPESP's process: 07/00216-2 - Temporal and spatial genes expression (Ras, Myc, Fos, Jun and Msx2) and their transcription factors regulated by matrix metalloproteinases, their tissue inhibitors and RECK during mouse endochondral ossification
• Grantee: Katiúcia Batista da Silva Paiva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 99/10655-5 - José Mauro Granjeiro | Faculdade de Odontologia de Bauru/USP - Brazil
• Grantee: Mari Cleide Sogayar
• Support Opportunities: Research Grants - Visiting Researcher Grant - Brazil
• FAPESP's process: 07/04148-1 - Temporal and spatial genes expression (Ras, Myc, Fos, Jun and Msx2) and their transcription factors regulated by matrix metalloproteinases, their tissue inhibitors, and RECK during mouse endochondral ossification
• Grantee: Fabio Daumas Nunes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 01/10707-7 - Molecular bases of the control of cell proliferation and origin of neoplasms in the genomic and proteomic era
• Grantee: Mari Cleide Sogayar
• Support Opportunities: Research Projects - Thematic Grants