Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lopap: A non-inflammatory and cytoprotective molecule in neutrophils and endothelial cells

Full text
Author(s):
Waismam, Kaline [1] ; Chudzinski-Tavassi, Ana Marisa [2] ; Carrijo-Carvalho, Linda C. [2] ; Fernandes Pacheco, Mario T. [1] ; Farsky, Sandra H. P. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, BR-05503900 Sao Paulo - Brazil
[2] Butantan Inst, Biochem & Biophys Lab, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Toxicon; v. 53, n. 6, p. 652-659, MAY 2009.
Web of Science Citations: 7
Abstract

Lopap (Lonomia obliqua prothrombin activator protease) is a member of the lipocalin family isolated from the extract of L obliqua bristles. Lopap displays serine protease-like activities, including coagulation disturbance, cytokine secretion and antiapoptotic activity in human cultured endothelial cells. Here, we have investigated the effects of the recombinant protein (rLopap) on the inflammatory and apoptotic processes of neutrophils and endothelial cells from male Wistar rats. We found that rLopap did not induce in vivo leukocyte-endothelial interactions in the microvasculature, initial steps of leukocyte recruitment during inflammation. Incubation of rLopap with neutrophils or endothelial cells prevented apoptosis evoked by serum deprivation and induced nitric oxide (NO) production in both cell types, and increased the expression of ICAM-1 by endothelial cells. Simultaneous incubation of endothelial cells or neutrophils with rLopap and N(omega)-nitro-L-arginine methyl ester (L-NAME), a non-specific inhibitor of NO synthases, inhibited NO production and impaired the protection on apoptosis. Differently, incubation of endothelial cells with monoclonal antibody anti ICAM-1 did not change the protection on apoptosis evoked by rLopap. Together, these results indicate that rLopap does not display inflammatory properties in vivo but inhibits apoptosis of neutrophils and endothelial cells depending, at least in part, on NO production. (C) 2009 Elsevier Ltd. All rights reserved. (AU)