Lipopeptides Produced by a Soil Bacillus Megaterium Strain

Author(s)

Pueyo, Manuel Troyano ^[1] ; Bloch, Jr., Carlos ^{[2, 3]} ; Carmona-Ribeiro, Ana Maria ^[1] ; di Mascio, Paolo ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil

^[2] LaboratA, Rio Espectrometria Massa, EMBRAPA, Recursos Gen, Brasilia, DF - Brazil

^[3] EMBRAPA, Lab Espectrometria Massa, Brasilia, DF - Brazil

• Document type: Journal article
• Source: MICROBIAL ECOLOGY; v. 57, n. 2, p. 367-378, FEB 2009.
• Web of Science Citations: 33

Abstract

A soil microorganism identified as Bacillum megaterium was found to produce several antibiotics substances after growth for 20 h at 37A degrees C in a mineral culture medium. Analysis both by electron spray ionization (ESI) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) identified these substances as lipopeptides. Predominant peaks at m/z 1,041 and m/z 1,065 revealed ions which are compatible with surfactins and lichenysins, respectively. Two other ions m/z 1,057 and m/z 1,464 were further studied by collision-induced dissociation (CID) unveiling an iturin A at the first and fengycins A and B at the second m/z peaks. The CID spectrum of the m/z 1,464 ion also suggests the existence of fengycins A and B variants in which Ile was changed to Val in the position 10 of the peptide moiety. Raw mixtures of all these compounds were also assayed for antibiotic features. The data enlighten the unusual diversity of the lipopeptide mixture produced by a sole Bacillus species. (AU)

• FAPESP's process: 98/11480-1 - Interactions betwee membranes, biomolecules and surfaces
• Grantee: Ana Maria Carmona-Ribeiro
• Support Opportunities: Research Projects - Thematic Grants