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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

On-line identification of minor flavones from sugarcane juice by LC/UV/MS and post-column derivatization

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Author(s):
Colombo, Renata [1] ; Yariwake, Janete H. [1] ; Queiroz, Emerson F. [2] ; Ndjoko, Karine [2] ; Hostettmann, Kurt [2]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Geneva, Lab Pharmacognosie & Phytochim, Ecole Pharm Geneve Lausanne, CH-1211 Geneva 4 - Switzerland
Total Affiliations: 2
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 20, n. 9, p. 1574-1579, 2009.
Web of Science Citations: 12
Abstract

This work describes the on-line characterization of minor flavones from sugarcane (Saccharum officinarum) juice by high-performance liquid chromatography coupled to diode array UV detection and mass spectrometry (LC/UV/MS) using atmospheric pressure chemical ionization-collision-induced dissociation (APCI-CID-MS/MS) and post-column derivatization using UV shift reagents. HPLC-UV analysis with shift reagents provided information about the substitution pattern in the flavonoid skeleton and, combined with MS data, these techniques allowed for the on-line identification of five "garapa" flavones: luteolin-8-C-glucosyl-7-O-glucuronide; tricin-7-O-neohesperoside-4'-O-rhamnoside; tricin-7-O-methylglucuronate-4'-O-rhamnoside; tricin-7-O-methylglucuronide; swertisin, while four other compounds were partially identified as glycosylflavones. Only swertisin (7-O-methylapigenin-6-C-glucoside) was reported previously in sugarcane molasses. (AU)