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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of topical 0.2% Cyclosporine A on corneal neovascularization induced by xenologous amniotic membrane implantation into a corneal stroma micropocket

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Author(s):
Milani, Juliana F. [1] ; Barros, Paulo S. M. [1] ; Guerra, Jose L. [2] ; Brooks, Dennis E. [3]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, FMVZ, Lab Invest Oftalmol Comparat, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, FMVZ, Dept Patol, BR-05508900 Sao Paulo - Brazil
[3] Univ Florida, Coll Vet Med, Sect Comparat Ophthalmol, Dept Small Anim Clin Sci, Gainesville, FL 32610 - USA
Total Affiliations: 3
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 28, n. 8, p. 379-386, AUG 2008.
Web of Science Citations: 1
Abstract

The objective of the study was to evaluate the topical effects of 0.2% Cyclosporine A (CsA) on corneal neovascularization of rats following surgical implantation of equine amniotic membrane into a corneal stroma micropocket. The implantation of xenologous amniotic membrane was performed bilaterally in 90 rats. In the same day of the surgery each right eye started receiving topical CsA twice a day. The left eye received no medication and served as a control. The evaluation of corneal neovascularization was performed by computerized image analysis and histopathological evaluation at 1, 3, 7, 15, 30 and 60 days postoperatively. For the image analysis 10 animals were used per time period, and for the histopathological examination, five animals were used per time period. Image analysis found that corneal neovascularization began on the 3rd postoperative day, reached its peak on the 7th day, and then progressively and rapidly decreased. Statistic analysis indicated that neovascularization of the CsA treated eye on the 7th day was significantly higher than that observed in untreated eyes. On the 30th day, however, this pattern was reversed with the neovascularization observed in the CsA treated eyes declining to the low levels observed on the 3rd day. The degree of neovascularization in the untreated eyes on the 30th day declined to the baseline levels found on day 3 at the 60th day. Histopathological analysis indicated that deposition of collagen in the implanted tissue was completed by the 15th day. Therefore, we concluded that ( 1) equine amniotic membrane in rat corneal stroma produced an intense neovascularization until the 15th day postoperatively and then regressed, ( 2) deposition of collagen of the implanted tissue was completed on the 15th day postoperatively, and ( 3) use of CsA was associated with increase in the corneal neovascularization initially, followed by a quick and intense regression. (AU)