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Histological evaluation of the effect of copaiba oil (Copaifera multijuga Hayne) at a concentration of 1% on the healing of corneal ulcers caused by chemical burns in rats

Grant number: 15/17641-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2016
Effective date (End): July 31, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:Adriana Torrecilhas Jorge
Grantee:Isabelli Ricordi
Home Institution: Coordenadoria Adjunta de Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
Associated research grant:11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species, AP.TEM

Abstract

The copaiba oil has anti-inflammatory action, antibiotic, analgesic and healing, however, nothing is described in the literature regarding the use and availability in ophthalmology. Will be evaluated the effect of eye drops to copaiba oil-based (Copaifera multijuga Hayne) in corneal ulcers caused chemically. They will be used 24 Wistar rats (Rattus norvegicus, Wistar albino), males and 250-300 grams of body weight. After general anesthesia, ulcers will be made on the left eye with filter paper disc soaked in 1N sodium hydroxide, for 80 seconds. The animals will be divided into 4 groups with 8 animals each. The G1 will receive copaiba oil at 1%; GT Tween 80 solution at 5%; GE, Epitezan® and the GL, Lacrima plus® the 6-hour intervals, for 72 hours consecutive. After the treatments, the animals will be submitted to active euthanasia with sodium pentobarbital and your eye bulbs collected by subconjunctival enucleation technique. Following routine preparation for light microscopy and stained with HE, will be evaluated epithelium and the corneal stroma and anterior chamber, verifying the presence of anomalous deposition and spongiosis of the epithelium, inflammatory cells, neovascularization, edema, corneal scarring, fibrin, and haemorrhage.