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Full text | |
Author(s): |
Coggan, Jennifer Anne
[1]
;
Melville, Priscilla Anne
[1]
;
de Oliveira, Clair Motos
[2]
;
Faustino, Marcelo
[2]
;
Moreno, Andrea Micke
[1]
;
Benites, Nilson Roberti
[1]
Total Authors: 6
|
Affiliation: | [1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim VPS, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Anim Reprod, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 2
|
Document type: | Journal article |
Source: | Brazilian Journal of Microbiology; v. 39, n. 3, p. 477-483, JUL-SEP 2008. |
Web of Science Citations: | 16 |
Abstract | |
As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study also consisted of the counting of microorganisms colonies forming units in samples of intrauterine content, tests of antimicrobial susceptibility of the E. coli isolates and the histological examination of the uterus. E. coli was the most prevalent microorganism isolated (76.6%) and 120 strains (79.5%) were positive for sfa, 86 (56.9%) were positive for cnf, 87 (57.6%) were positive for pap, 52 (34.4%) were positive for hly, 51 (33.8%) were positive for iuc and 5 (3.3%) were positive for afa genes. One observed more sensitivity of E. coli to norfloxacin, polimixin B, sulphazotrin, chloranfenicol and enrofloxacin. In 42% of the samples of uterine walls where microorganisms were isolated, the sizes of the areas of the inflammatory responses corresponded to 39-56%. Virulence factors were identified in 98.0% of the strains evaluated, demonstrating a high frequency of potentially pathogenic E. coli. It must be considered that dogs are animals that are living in close proximity to man for thousands of years and have an important role in the transmission of E. coli to other animals and to man. (AU) |