Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Author(s)

Viana, D. A. ^[1] ; Carneiro-Cunha, M. N. ^[2] ; Araujo, J. M. ^[3] ; Barros-Neto, B. ^[4] ; Lima-Filho, J. L. ^[2] ; Converti, A. ^[5] ; Pessoa-Junior, A. ^[1] ; Porto, A. L. F. ^[6]

Affiliation

^[1] Univ Sao Paulo, Dept Pharmaceut Technol & Biochem, BR-05508000 Sao Paulo - Brazil

^[2] Univ Fed Pernambuco, LIKA, BR-50670901 Recife, PE - Brazil

^[3] Univ Fed Pernambuco, Dept Antibiot, BR-0740540 Recife, PE - Brazil

^[4] Univ Fed Pernambuco, Dept Fundamental Chem, BR-0740540 Recife, PE - Brazil

^[5] Univ Genoa, Dept Chem & Proc Engn, I-16145 Genoa - Italy

^[6] Univ Fed Rural Pernambuco, Dept Anim Morphol & Physiol, BR-52171900 Dois Irmaos, PE - Brazil

• Document type: Journal article
• Source: Applied Biochemistry and Biotechnology; v. 160, n. 6, p. 1797-1807, MAR 2010.
• Web of Science Citations: 10

Abstract

Clavulanic acid (CA) is a beta-lactam antibiotic, which has a potent beta-lactamase inhibiting activity. The influence of five variables, namely pH (6.0, 6.4, and 6.8), temperature (28A degrees C, 30A degrees C, and 32A degrees C), agitation intensity (150, 200, and 250 rpm), glycerol concentration (5.0, 7.5, and 10 g/L) and soybean flour concentration (5.0, 12.5, and 20 g/L), on CA production by a new isolate of Streptomyces (DAUFPE 3060) was investigated in 250-mL Erlenmeyer flasks using a fractional factorial design. Temperature and soybean flour concentration were shown to be the two variables that exerted the most important effects on the production of CA at 95% confidence level. The highest CA concentration (494 mg/L) was obtained after 48 h at 150 rpm, 32A degrees C, pH 6.0, 5.0 g/L glycerol, and 20 g/L soybean flour concentrations. Under these conditions, the yields of biomass and product on consumed substrate were 0.26 g(X)/g(S) and 64.3 mg(P)/g(S), respectively. Fermentations performed in 3.0-L bench-scale fermenter allowed increasing the CA production by about 60%. (AU)