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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recovery and upgrading bovine rumen protein by extrusion: Effect of lipid content on protein disulphide cross-linking, solubility and molecular weight

Full text
Author(s):
Vaz, L. C. M. A. [1] ; Areas, J. A. G. [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Lab Bioquim & Propriedades Funcionais Alimentos, Fac Saude Publ, BR-01246904 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: MEAT SCIENCE; v. 84, n. 1, p. 39-45, JAN 2010.
Web of Science Citations: 7
Abstract

Bovine rumen protein with two levels of residual lipids (1.9% or 3.8%) was subjected to thermoplastic extrusion under different temperatures and moisture contents. Protein Solubility in different buffers, disulphide cross-linking and molecular weight distribution were determined on the extrudates. After extrusion, samples with 1.9% residual lipids content had a higher concentration of protein insoluble by undetermined forces, irrespective of feed moisture and processing temperature used. Lipid content of 3.8% in the feed material resulted in more protein participating in the extrudate network through non-covalent interactions (hydrophobic and electrostatic) and disulphide bonds. A small dependency of the extrusion process on moisture and temperature and a marked dependency on lipid content, especially phospholipid, was observed, Electrophoresis under non-reducing conditions showed that protein extrusion with low feed moisture promoted high molecular breakdown inside the barrel, probably due to intense shear force, and further protein aggregation at the die end. (C) 2009 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 07/03896-4 - Protein isolate from amaranthus (Amaranthus cruentus L, BRS-Alegria): functional properties of the protein and identification of bioactive peptides
Grantee:José Alfredo Gomes Arêas
Support Opportunities: Regular Research Grants