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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of auxin and gibberellin in citrus canker development and in the transcriptional control of cell-wall remodeling genes modulated by Xanthomonas axonopodis pv. citri

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Author(s):
Cernadas, Raul Andres [1] ; Benedetti, Celso Eduardo [1]
Total Authors: 2
Affiliation:
[1] Brazilian Synchrotron Light Lab, Ctr Mol & Struct Biol, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Plant Science; v. 177, n. 3, p. 190-195, SEP 2009.
Web of Science Citations: 24
Abstract

Xanthomonas axonopodis pv. citri (Xac) is the causal agent of citrus canker, a disease characterized by hyperplastic lesions on the host surface. We have shown previously that Xac strongly increases transcription of sweet orange (Citrus sinensis) genes encoding cell-wall remodeling enzymes and proteins associated with cell division and expansion. In addition, a number of Xac-regulated genes are implicated in the synthesis, mobilization and signaling of auxin and gibberellin. Since auxin and gibberellin control cell division and elongation and are known to modulate the expression of cell-wall hydrolytic enzymes, we examined their ability to modulate the transcription of the cell-wall remodeling genes altered by Xac. Indeed, the expression of various citrus cellulases, pectinesterases, expansin and galactosyltransferase was rapidly and similarly affected by auxin and gibberellin. Moreover, the changes in transcript levels of the cell-wall remodeling genes mediated by auxin and gibberellin correlated with changes in the expression of genes involved in gibberellin synthesis, auxin signaling and mobilization, as observed during Xac infection. Most significantly, auxin promoted canker development whereas an inhibitor of auxin transport reduced pustule formation. Interestingly, an inhibitor of gibberellin synthesis significantly inhibited the auxin-induced transcription, as well as the appearance of canker lesions, thus suggesting that a crosstalk between auxin and gibberellin controls the transcription of orange genes involved in cell division and expansion trigged by Xac. (C) 2009 Elsevier Ireland Ltd. All rights reserved. (AU)

FAPESP's process: 00/10266-8 - A structural biology laboratory network for the study of the 3D structures of proteins
Grantee:Nilson Ivo Tonin Zanchin
Support Opportunities: Genome Research Grants
FAPESP's process: 98/14138-2 - Center for Structural Molecular Biotechnology
Grantee:Glaucius Oliva
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC