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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression of eight genes of nuclear factor-kappa B pathway in multiple myeloma using bone marrow aspirates obtained at diagnosis

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Author(s):
Sampaio Almeida, Manuella S. ; Vettore, Andre L. [1, 2] ; Yamamoto, Mihoko ; Chauffaille, Maria de Lourdes L. F. ; Zago, Marco Antonio [3] ; Colleoni, Gisele W. B. [4]
Total Authors: 6
Affiliation:
[1] Ludwig Inst Canc Res, Sao Paulo Branch, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Biol, UNIFESP, BR-04023900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, UNIFESP, EPM, Disciplina Hematol & Hemoterapia, Dept Hematol & Hemotherapy, BR-04023900 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: HISTOLOGY AND HISTOPATHOLOGY; v. 24, n. 8, p. 991-997, AUG 2009.
Web of Science Citations: 8
Abstract

Purpose: To evaluate the expression of NF-kappa B pathway genes in total bone marrow samples obtained from MM at diagnosis using real-time quantitative PCR and to evaluate its possible correlation with disease clinical features and survival. Material and methods: Expression of eight genes related to NF-kappa B pathway (NFKB1, IKB, RANK, RANKL, OPG, IL6, VCAM1 and ICAM1) were studied in 53 bone marrow samples from newly diagnosed MM patients and in seven normal controls, using the Taqman system. Genes were considered overexpressed when tumor expression level was at least four times higher than that observed in normal samples. Results: The percentages of overexpression of the eight genes were: NFKB1 0%, IKB 22.6%, RANK 15.1%, RANKL 31.3%, OPG 7.5%, IL6 39.6%, VCAM1 10% and ICAM1 26%. We found association between IL6 expression level and International Staging System (ISS) (p = 0.01), meaning that MM patients with high ISS scores have more chance of overexpression of IL6. The mean value of ICAM1 relative expression was also associated with the ISS score (p = 0.02). Regarding OS, cases with IL6 overexpression present worse evolution than cases with IL6 normal expression (p = 0.04). Conclusion: We demonstrated that total bone marrow aspirates can be used as a source of material for gene expression studies in MM. In this context, we confirmed that IL6 overexpression was significantly associated with worse survival and we described that it is associated with high ISS scores. Also, ICAM1 was overexpressed in 26% of cases and its level was associated with ISS scores. (AU)