| Full text | |
| Author(s): |
Cabral, H.
;
Leopoldino, A. M.
;
Tajara, E. H.
;
Greene, L. J.
;
Faca, V. M.
;
Mateus, R. P.
;
Ceron, C. R.
;
Souza Judice, W. A. de
;
Juliano, L.
;
Bonilla-Rodriguez, G. O.
[10]
Total Authors: 10
|
| Document type: | Journal article |
| Source: | PROTEIN AND PEPTIDE LETTERS; v. 13, n. 1, p. 83-89, Jan. 2006. |
| Field of knowledge: | Biological Sciences - Biochemistry |
| Abstract | |
The present work reports the characterization of Fastuosain, a novel cysteine protease of 25kDa, purified from the unripe fruits of Bromelia fastuosa, a wild South American Bromeliaceae. Proteolytic activity, measured using casein and synthetic substrates, was dependent on the presence of thiol reagents, having maximum activity at pH 7.0. The present work reports cDNA cloning of Fastuosain; cDNA was amplified by PCR using specific primers. The product was 1096pb long. Mature fastuosain has 217 residues, and with the proregion has a total length of 324 residues. Its primary sequence showed high homology with ananain(74%), stem bromelain (66%) and papain (44%). (AU) | |
| FAPESP's process: | 98/05967-5 - Purificacao de cisteino-proteases de bromelia fastuosa (gravata). |
| Grantee: | Hamilton Cabral |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 00/04932-5 - Estrutura-função de proteínas: hemoglobinas e proteinases |
| Grantee: | Gustavo Orlando Bonilla Rodriguez |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 03/00085-4 - Estrutura-função em proteínas: 1. enzimas proteolíticas com potencial biotecnológico e 2. hemoglobinas animais |
| Grantee: | Gustavo Orlando Bonilla Rodriguez |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 01/03912-3 - Caracterizacao estrutural e funcional e uma proteinase tiolica de frutos de bromelia fastuosa: analise funcional e estrutural comparativa com papaina e bromelinas. |
| Grantee: | Hamilton Cabral |
| Support Opportunities: | Scholarships in Brazil - Doctorate |