Radiopacifiers do not induce genetic damage in murine fibroblasts: an in vitro study

Author(s)

Ribeiro, D. A. ^[1] ; Carlin, V. ^[2] ; Fracalossi, A. C. C. ^[2] ; Oyama, L. M. ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Biociencias, BR-11060001 Santos, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Pathol, BR-11060001 Santos, SP - Brazil

• Document type: Journal article
• Source: International Endodontic Journal; v. 42, n. 11, p. 987-991, 2009.
• Web of Science Citations: 10

Abstract

P>Aim To evaluate whether several radiopacifiers are able to induce genetic damage in a laboratory cell culture study. Methodology Murine fibroblasts were exposed to barium sulphate, bismuth oxide or zirconium oxide, at final concentrations ranging from 10 to 1 000 mu g mL-1 for 1 h at 37 degrees C. The negative control group was treated with a vehicle control [phosphate buffered solution (PBS)] for 1 h at 37 degrees C and the positive control group was treated with hydrogen peroxide (at 10 mu M) for 5 min on ice. Genotoxicity data were assessed by the single-cell gel (comet) assay. Results All the tested compounds did not induce DNA breakage as depicted by the mean tail moment in all the concentrations analysed. Conclusion Exposure to the tested radiopacifiers may not be a factor that increases the level of DNA lesions in mammalian cells as detected by a single-cell gel (comet) assay. (AU)

• FAPESP's process: 07/01228-4 - Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis
• Grantee: Daniel Araki Ribeiro
• Support Opportunities: Research Grants - Young Investigators Grants