| Full text | |
| Author(s): Show less - |
Brohem, Carla A.
[1]
;
Massaro, Renato R.
[1]
;
Tiago, Manoela
[1]
;
Marinho, Camila E.
[1]
;
Jasiulionis, Miriam G.
[2]
;
de Almeida, Rebeca L.
[1]
;
Rivelli, Diogo P.
[1]
;
Albuquerque, Renata C.
[1]
;
de Oliveira, Tiago F.
[1]
;
de Melo Loureiro, Ana P.
[1]
;
Okada, Sabrina
[1]
;
Soengas, Maria S.
[3]
;
de Moraes Barros, Silvia B.
[1]
;
Maria-Engler, Silvya S.
[1]
Total Authors: 14
|
| Affiliation: | [1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin Chem & Toxicol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Pharmacol, Sao Paulo - Brazil
[3] Spanish Natl Canc Res Ctr, Ctr Nacl Invest Oncol, Madrid - Spain
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | PIGMENT CELL & MELANOMA RESEARCH; v. 25, n. 3, p. 354-369, MAY 2012. |
| Web of Science Citations: | 27 |
| Abstract | |
Induction of apoptotic cell death in response to chemotherapy and other external stimuli has proved extremely difficult in melanoma, leading to tumor progression, metastasis formation and resistance to therapy. A promising approach for cancer chemotherapy is the inhibition of proteasomal activity, as the half-life of the majority of cellular proteins is under proteasomal control and inhibitors have been shown to induce cell death programs in a wide variety of tumor cell types. 4-Nerolidylcatechol (4-NC) is a potent antioxidant whose cytotoxic potential has already been demonstrated in melanoma tumor cell lines. Furthermore, 4-NC was able to induce the accumulation of ubiquitinated proteins, including classic targets of this process such as Mcl-1. As shown for other proteasomal inhibitors in melanoma, the cytotoxic action of 4-NC is time-dependent upon the pro-apoptotic protein Noxa, which is able to bind and neutralize Mcl-1. We demonstrate the role of 4-NC as a potent inducer of ROS and p53. The use of an artificial skin model containing melanoma also provided evidence that 4-NC prevented melanoma proliferation in a 3D model that more closely resembles normal human skin. (AU) | |
| FAPESP's process: | 10/50157-5 - Impacto da expressao de reck no controle da invasao de melanoma: estudo em monocamadas e pele artificial |
| Grantee: | Carla Abdo Brohem |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 08/58817-4 - Generation of human artificial skins and invasive melanomas as a platform for pharmacological testing |
| Grantee: | Silvya Stuchi Maria-Engler |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 06/50479-7 - Action of Pothomorphe umbellata extract on the extracellular matrix components in the skin healing process: in vivo and in vitro models |
| Grantee: | Carla Abdo Brohem |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 06/60930-8 - Pesquisa sistematica de plantas medicinais: potencial farmacoterapeutico de pothomorphe umbellata. |
| Grantee: | Silvia Berlanga de Moraes Barros |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 09/54816-6 - Xenobiotic effects in cultured human cells and study of mechanisms of cytotoxic and genotoxic damage |
| Grantee: | Ana Paula de Melo Loureiro |
| Support Opportunities: | Regular Research Grants |